Oxo-Functionalized Graphene as a Cell Membrane Carrier of Nucleic Acid Probes Controlled by Aging
Artikel i vetenskaplig tidskrift, 2016

We applied a fluorescein-containing oligonucleotide ( ON) to probe surface properties of oxidized graphene ( oxo-G) and observed that graphene-like patches are formed upon aging of oxo-G, indicated by enhanced probe binding and by FTIR spectroscopic analysis. By using a recently developed fluorogenic endoperoxide ( EP) probe, we confirmed that during the aging process the amount of EPs on the oxo-G surface is reduced. Furthermore, aging was found to strongly affect cell membrane carrier properties of this material. In particular, freshly prepared oxo-G does not act as a carrier, whereas oxo-G aged for 28 days at 4 degrees C is an excellent carrier. Based on these data we prepared an optimized oxo-G, which has a low-defect density, binds ONs, is not toxic, and acts as cell membrane carrier. We successfully applied this material to design fluorogenic probes of representative intracellular nucleic acids 28S rRNA and beta-actin-mRNA. The results will help to standardize oxidized graphene derivatives for biomedical and bioanalytical applications.

carbon framework

dna

graphene oxide

oxide

aging

spectroscopy

oligonucleotides

mechanisms

reduction

stability

spectroscopy

oxo-functionalized graphene

challenges

nano-graphene

Chemistry

origin

Författare

H. Pieper

Friedrich-Alexander-Universität Erlangen Nurnberg (FAU)

C. E. Halbig

Friedrich-Alexander-Universität Erlangen Nurnberg (FAU)

L. Kovbasyuk

Friedrich-Alexander-Universität Erlangen Nurnberg (FAU)

M. R. Filipovic

Institut Biochimie et Genetique Cellulaires, Bordeaux

Siegfried Eigler

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Organisk kemi

A. Mokhir

Friedrich-Alexander-Universität Erlangen Nurnberg (FAU)

Chemistry - A European Journal

0947-6539 (ISSN) 1521-3765 (eISSN)

Vol. 22 43 15389-15395

Styrkeområden

Nanovetenskap och nanoteknik

Ämneskategorier

Fysikalisk kemi

Nanoteknik

DOI

10.1002/chem.201603063