The bacterial curli system possesses a potent and selective inhibitor of amyloid formation.
Artikel i vetenskaplig tidskrift, 2015
Curli are extracellular functional amyloids that are assembled by enteric bacteria during biofilm formation and host colonization. An efficient secretion system and chaperone network ensures that the major curli fiber subunit, CsgA, does not form intracellular amyloid aggregates. We discovered that the periplasmic protein CsgC was a highly effective inhibitor of CsgA amyloid formation. In the absence of CsgC, CsgA formed toxic intracellular aggregates. In vitro, CsgC inhibited CsgA amyloid formation at substoichiometric concentrations and maintained CsgA in a non-β-sheet-rich conformation. Interestingly, CsgC inhibited amyloid assembly of human α-synuclein, but not Aβ42, in vitro. We identified a common D-Q-Φ-X0,1-G-K-N-ζ-E motif in CsgC client proteins that is not found in Aβ42. CsgC is therefore both an efficient and selective amyloid inhibitor. Dedicated functional amyloid inhibitors may be a key feature that distinguishes functional amyloids from disease-associated amyloids.
Amyloid beta-Peptides
Escherichia coli
metabolism
alpha-Synuclein
Secondary
chemistry
metabolism
metabolism
drug effects
Escherichia coli Proteins
genetics
Protein Aggregates
In Vitro Techniques
Humans
Base Sequence
chemistry
Protein Structure
metabolism
genetics
Molecular Sequence Data
pharmacology
Amino Acid Motifs
Författare
Margery L Evans
University of Michigan
E. Chorell
Umeå universitet
Jonathan D Taylor
Imperial College London
J. Åden
Umeå universitet
Anna Götheson
Umeå universitet
Fei Li
University of Michigan
Marion Koch
Imperial College London
Lea Sefer
Imperial College London
Steve J Matthews
Imperial College London
Pernilla Wittung Stafshede
Umeå universitet
F. Almqvist
Umeå universitet
Matthew R Chapman
Umeå universitet
University of Michigan
Molecular Cell
1097-2765 (ISSN) 1097-4164 (eISSN)
Vol. 57 3 445-55Ämneskategorier
Cellbiologi
Biokemi och molekylärbiologi
Biofysik
DOI
10.1016/j.molcel.2014.12.025
PubMed
25620560