Identification of New Potential Interaction Partners for Human Cytoplasmic Copper Chaperone Atox1: Roles in Gene Regulation?
Artikel i vetenskaplig tidskrift, 2015

The human copper (Cu) chaperone Atox1 delivers Cu to P1B type ATPases in the Golgi network, for incorporation into essential Cu-dependent enzymes. Atox1 homologs are found in most organisms; it is a 68-residue ferredoxin-fold protein that binds Cu in a conserved surface-exposed Cys-X-X-Cys (CXXC) motif. In addition to its well-documented cytoplasmic chaperone function, in 2008 Atox1 was suggested to have functionality in the nucleus. To identify new interactions partners of Atox1, we performed a yeast two-hybrid screen with a large human placenta library of cDNA fragments using Atox1 as bait. Among 98 million fragments investigated, 25 proteins were found to be confident interaction partners. Nine of these were uncharacterized proteins, and the remaining 16 proteins were analyzed by bioinformatics with respect to cell localization, tissue distribution, function, sequence motifs, three-dimensional structures and interaction networks. Several of the hits were eukaryotic-specific proteins interacting with DNA or RNA implying that Atox1 may act as a modulator of gene regulation. Notably, because many of the identified proteins contain CXXC motifs, similarly to the Cu transport reactions, interactions between these and Atox1 may be mediated by Cu.

Models

Computational Biology

Humans

Databases

Gene Expression Regulation

metabolism

Two-Hybrid System Techniques

Molecular

Tertiary

Metallochaperones

chemistry

chemistry

Molecular Chaperones

metabolism

Copper

Protein Interaction Mapping

Protein Structure

Cytoplasm

metabolism

Protein Binding

Protein

metabolism

Författare

[Person efe18ab8-eaac-4b12-b432-6505229f5484 not found]

Uppsala universitet

[Person 5b336542-7b04-4293-9b9f-9bd0e136c94e not found]

Umeå universitet

International Journal of Molecular Sciences

16616596 (ISSN) 14220067 (eISSN)

Vol. 16 8 16728-39

Ämneskategorier

Biologisk systematik

Cellbiologi

Biologiska vetenskaper

Biofysik

DOI

10.3390/ijms160816728

PubMed

26213915

Mer information

Senast uppdaterat

2019-06-12