Comparative Systems Analyses Reveal Molecular Signatures of Clinically tested Vaccine Adjuvants
Artikel i vetenskaplig tidskrift, 2016

A better understanding of the mechanisms of action of human adjuvants could inform a rational development of next generation vaccines for human use. Here, we exploited a genome wide transcriptomics analysis combined with a systems biology approach to determine the molecular signatures induced by four clinically tested vaccine adjuvants, namely CAF01, IC31, GLA-SE and Alum in mice. We report signature molecules, pathways, gene modules and networks, which are shared by or otherwise exclusive to these clinical-grade adjuvants in whole blood and draining lymph nodes of mice. Intriguingly, co-expression analysis revealed blood gene modules highly enriched for molecules with documented roles in T follicular helper (TFH) and germinal center (GC) responses. We could show that all adjuvants enhanced, although with different magnitude and kinetics, TFH and GC B cell responses in draining lymph nodes. These results represent, to our knowledge, the first comparative systems analysis of clinically tested vaccine adjuvants that may provide new insights into the mechanisms of action of human adjuvants.

t-cell responses

immunity

Science & Technology - Other Topics

activation

dendritic cells

alum adjuvant

generation

analysis

tuberculosis vaccine

expression

infection

network

Författare

T. A. Olafsdottir

Göteborgs universitet

M. Lindqvist

Göteborgs universitet

Intawat Nookaew

Chalmers, Biologi och bioteknik, Systembiologi

P. Andersen

Statens Serum Institut

J. Maertzdorf

Max Planck-institutet

J. Persson

Göteborgs universitet

D. Christensen

Statens Serum Institut

Y. Zhang

Göteborgs universitet

J. Anderson

Göteborgs universitet

Sakda Khoomrung

Chalmers, Biologi och bioteknik, Systembiologi

Partho Sen

Chalmers, Biologi och bioteknik, Systembiologi

E. M. Agger

Statens Serum Institut

R. Coler

Infectious Disease Research Institute

D. Carter

Infectious Disease Research Institute

A. Meinke

Vienna Biocenter

R. Rappuoli

GSK Vaccines

S. H. E. Kaufmann

Max Planck-institutet

S. G. Reed

Infectious Disease Research Institute

A. M. Harandi

Göteborgs universitet

Scientific Reports

2045-2322 (ISSN)

Vol. 6 39097

Ämneskategorier

Biokatalys och enzymteknik

Organisk kemi

DOI

10.1038/srep39097