Asymmetric cationic liposomes designed for heat-activated association with cells
Artikel i vetenskaplig tidskrift, 2017

Improved anticancer drugs and drug carriers are needed in combination therapies, such as hyperthermia-assisted chemotherapy. Liposomal drug carriers with advanced functions are attractive candidates for targeted accumulation and drug release in response to heat stimulus. We report on the design of liposomes with a heat-activated surface function. Our design is based on asymmetric lipid membranes with a defined gel to liquid-crystalline phase-transition temperature around 41 °C. Asymmetry between the inner and the outer membrane leaflets was generated through selective PEGylation of cationic lipids in the outer membrane leaflet. In a physiological buffer, the PEGylated asymmetric liposomes had a neutral zeta potential and did not bind to planar anionic model membranes. In contrast, following upon heat-activation, binding of liposomes to the model membranes occurred. Release of a hydrophilic dye encapsulated in the asymmetric liposomes occurred at 40 °C. Enhanced uptake of the asymmetric liposomes by hypopharyngeal carcinoma cells (FaDu cells) was observed when hyperthermia was applied compared to experiments performed at 37 °C. These results show the potential of asymmetric liposomes for localized delivery of drugs into cells in response to (external) temperature stimulus.

Membrane asymmetry

Cellular uptake

Cationic liposomes

Hyperthermia

PEGylation

Författare

Yujia Jing

Chalmers, Fysik, Biologisk fysik

Anna Danielsson

Sahlgrenska akademin

Hana Dobsicek Trefna

Chalmers, Signaler och system, Signalbehandling och medicinsk teknik, Biomedicinsk elektromagnetik

Mikael Persson

Chalmers, Signaler och system, Signalbehandling och medicinsk teknik, Biomedicinsk elektromagnetik

Sofia Svedhem

Chalmers, Fysik, Biologisk fysik

Colloids and Surfaces B: Biointerfaces

0927-7765 (ISSN)

Vol. 151 112-118

Ämneskategorier

Fysik

Elektroteknik och elektronik

DOI

10.1016/j.colsurfb.2016.06.041

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Senast uppdaterat

2018-03-23