HAG2/MYB76 and HAG3/MYB29 exert a specific and coordinated control on the regulation of aliphatic glucosinolate biosynthesis in Arabidopsis thaliana
Artikel i vetenskaplig tidskrift, 2008

In a previous transactivation screen, two Arabidopsis thaliana R2R3-MYB transcription factors, HAG2/MYB76 and HAG3/MYB29, along with the already characterized HAG1/MYB28, were identified as putative regulators of aliphatic glucosinolate biosynthesis. • Molecular and biochemical characterization of HAG2/MYB76 and HAG3/MYB29 functions was performed using transformants with increased or repressed transcript levels. Real-time PCR assays, cotransformation assays and measurements of glucosinolate contents were used to assess the impact of both MYB factors on the steady-state level of glucosinolate biosynthetic genes and accumulation of aliphatic glucosinolates. • Both HAG2/MYB76 and HAG3/MYB29 were shown to be positive regulators of aliphatic glucosinolate biosynthesis. Expression of promoter-β- glucuronidase (GUS) fusions indicated GUS activities in both vegetative and generative organs, with distinct characteristics for each MYB factor. HAG1/MYB28, HAG2/MYB76 and HAG3/MYB29 reciprocally transactivated each other in the control of aliphatic glucosinolate biosynthesis and downregulated the expression of genes involved in the control of indolic glucosinolate biosynthesis, pointing to a reciprocal negative regulation of these two pathways. • All three HAG transcription factors exert a coordinated control on aliphatic glucosinolate biosynthesis.

Methyl jasmonate-responsive genes

Glucosinolates

MYB transcription factors

Regulatory network

Transactivation

Författare

T. Gigolashvili

Martin Engqvist

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

R. Yatusevich

C. Müller

U.I. Flügge

New Phytologist

0028-646X (ISSN) 1469-8137 (eISSN)

Vol. 177 627-642

Ämneskategorier

Botanik

Biokemi och molekylärbiologi

DOI

10.1111/j.1469-8137.2007.02295.x