Vitamin C Pretreatment Enhances the Antibacterial Effect of Cold Atmospheric Plasma
Artikel i vetenskaplig tidskrift, 2017

Bacterial biofilms are three-dimensional structures containing bacterial cells enveloped in a protective polymeric matrix, which renders them highly resistant to antibiotics and the human immune system. Therefore, the capacity to make biofilms is considered as a major virulence factor for pathogenic bacteria. Cold Atmospheric Plasma (CAP) is known to be quite efficient in eradicating planktonic bacteria, but its effectiveness against biofilms has not been thoroughly investigated. The goal of this study was to evaluate the effect of exposure of CAP against mature biofilm for different time intervals and to evaluate the effect of combined treatment with vitamin C. We demonstrate that CAP is not very effective against 48 h mature bacterial biofilms of several common opportunistic pathogens: Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa. However, if bacterial biofilms are pre-treated with vitamin C for 15 min before exposure to CAP, a significantly stronger bactericidal effect can be obtained. Vitamin C pretreatment enhances the bactericidal effect of cold plasma by reducing the viability from 10 to 2% in E. coli biofilm, 50 to 11% in P. aeruginosa, and 61 to 18% in S. epidermidis biofilm. Since it is not feasible to use extended CAP treatments in medical practice, we argue that the pre-treatment of infectious lesions with vitamin C prior to CAP exposure can be a viable route for efficient eradication of bacterial biofilms in many different applications.

cold plasma

vitamin C

anti bacterial

biofilm

resistance

Författare

Saga Huld Helgadóttir

Chalmers, Fysik

Santosh Pandit

Chalmers, Biologi och bioteknik, Systembiologi

Venkata Raghu Mokkapati

Chalmers, Biologi och bioteknik, Systembiologi

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

Peter Apell

Chalmers, Fysik, Kemisk fysik

Ivan Mijakovic

Chalmers, Biologi och bioteknik, Systembiologi

Frontiers in cellular and infection microbiology

2235-2988 (ISSN)

Vol. 7 FEB 43

Ämneskategorier

Mikrobiologi

Mikrobiologi inom det medicinska området

Styrkeområden

Livsvetenskaper och teknik

DOI

10.3389/fcimb.2017.00043

PubMed

28275584