Increased lipid accumulation and adipogenic gene expression of adipocytes in 3D bioprinted nanocellulose scaffolds
Artikel i vetenskaplig tidskrift, 2017
Compared to standard 2D culture systems, new methods for 3D cell culture of adipocytes could provide more physiologically accurate data and a deeper understanding of metabolic diseases such as diabetes. By resuspending living cells in a bioink of nanocellulose and hyaluronic acid, we were able to print 3D scaffolds with uniform cell distribution. After one week in culture, cell viability was 95%, and after two weeks the cells displayed a more mature phenotype with larger lipid droplets than standard 2D cultured cells. Unlike cells in 2D culture, the 3D bioprinted cells did not detach upon lipid accumulation. After two weeks, the gene expression of the adipogenic marker genes PPAR. and FABP4 was increased 2.0- and 2.2-fold, respectively, for cells in 3D bioprinted constructs compared with 2D cultured cells. Our 3D bioprinted culture system produces better adipogenic differentiation of mesenchymal stem cells and a more mature cell phenotype than conventional
adipose tissue
model
bioink
constructs
adipose-tissue
stem-cells
differentiation
3D culture
Supplementary material for
Engineering
adipocyte
bacterial cellulose
culture
Materials Science
preadipocytes
nanocellulose bioink
gelatin
3D bioprinting
Författare
Ida Henriksson
Chalmers, Kemi och kemiteknik, Tillämpad kemi
Paul Gatenholm
Chalmers, Kemi och kemiteknik, Tillämpad kemi
Daniel Hägg
Chalmers, Kemi och kemiteknik, Tillämpad kemi
Göteborgs universitet
Biofabrication
1758-5082 (ISSN) 1758-5090 (eISSN)
Vol. 9 1 Article Number: 015022 - 015022Fundament
Grundläggande vetenskaper
Styrkeområden
Livsvetenskaper och teknik (2010-2018)
Ämneskategorier
Kemi
DOI
10.1088/1758-5090/aa5c1c