Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink
Artikel i vetenskaplig tidskrift, 2017

Cartilage lesions can progress into secondary osteoarthritis and cause severe clinical problems in numerous patients. As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes. Two bioinks were investigated: NFC with alginate (NFC/A) or hyaluronic acid (NFC/HA). Low proliferation and phenotypic changes away from pluripotency were seen in the case of NFC/HA. However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs. Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing. We conclude that NFC/A bioink is suitable for bioprinting iPSCs to support cartilage production in co-cultures with irradiated chondrocytes.

Bone

Human

Chondrogenic Differentiation

Hydrogel

Knee

Autologous Chondrocyte Implantation

System

Self-Renewal

Articular Chondrocytes

Pluripotent Stem-Cells

Författare

Duy Nguyen

Chalmers, Kemi och kemiteknik, Kemiteknik, Kemisk miljövetenskap

Daniel Hägg

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Polymerteknologi

Alma Forsman

Göteborgs universitet

Josefine Ekholm

Göteborgs universitet

P. Nimkingratana

Sahlgrenska akademin

Camilla Brantsing

Göteborgs universitet

Theodoros Kalogeropoulos

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Polymerteknologi

Samantha Zaunz

Chalmers, Kemi och kemiteknik

Sebastian Concaro

Göteborgs universitet

Mats Brittberg

Göteborgs universitet

Anders Lindahl

Göteborgs universitet

Paul Gatenholm

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Polymerteknologi

Annika Enejder

Chalmers, Biologi och bioteknik, Kemisk biologi

Stina Simonsson

Göteborgs universitet

Scientific Reports

2045-2322 (ISSN)

Vol. 7 00690

Ämneskategorier

Biomaterialvetenskap

DOI

10.1038/s41598-017-00690-y