Expression of NADH-oxidases enhances ethylene productivity in Saccharomyces cerevisiae expressing the bacterial EFE
Artikel i vetenskaplig tidskrift, 2017

Ethylene is a major petrochemical for which biotechnological production methods are an attractive alternative. Here we use a system based on a bacterial ethylene forming enzyme (EFE) expressed in Saccharomyces cerevisiae. Metabolic modelling performed in a previous study identified re-oxidation of NADH as a factor limiting ethylene production in S. cerevisiae. In line with this, we here found that strains with multicopy plasmid expression of the heterologous oxidases nox and Aox1 led to significantly increased specific ethylene productivity, up 12 and 36%, respectively, compared to the control strain with empty plasmid. However the productivity and yield was only improved in the AOX expressing strain compared to that of the control strain. Both oxidase expressing strains also exhibited increased respiration rates compared to the reference strain, with specific oxygen consumption rates being roughly doubled in both strains. The AOX strain furthermore exhibited a significant increase in the EFE substrate 2-oxoglutarate formation compared to the reference strain, linking an improvement in ethylene production to both increased respiratory capacity and increased substrate availability, thereby corroborating our previous finding.

2-oxoglutarate

Saccharomyces cerevisiae

in silico metabolic modelling

oxidase

ethylene

respiration

Författare

Nina Johansson

Chalmers, Biologi och bioteknik, Systembiologi

Karl Persson

Göteborgs universitet

Joakim Norbeck

Chalmers, Biologi och bioteknik, Systembiologi

Christer Larsson

Chalmers, Biologi och bioteknik, Systembiologi

Biotechnology and Bioprocess Engineering

1226-8372 (ISSN)

Vol. 22 2 195-199

Ämneskategorier

Industriell bioteknik

DOI

10.1007/s12257-016-0602-x

Mer information

Senast uppdaterat

2018-02-21