Cadmium Causes Misfolding and Aggregation of Cytosolic Proteins in Yeast
Artikel i vetenskaplig tidskrift, 2017

Cadmium is a highly poisonous metal and is classified as a human carcinogen. While its toxicity is undisputed, the underlying in vivo molecular mechanisms are not fully understood. Here, we demonstrate that cadmium induces aggregation of cytosolic proteins in living Saccharomyces cerevisiae cells. Cadmium primarily targets proteins in the process of synthesis or folding, probably by interacting with exposed thiol groups in not-yet-folded proteins. On the basis of in vitro and in vivo data, we show that cadmium-aggregated proteins form seeds that increase the misfolding of other proteins. Cells that cannot efficiently protect the proteome from cadmium-induced aggregation or clear the cytosol of protein aggregates are sensitized to cadmium. Thus, protein aggregation may contribute to cadmium toxicity. This is the first report on how cadmium causes misfolding and aggregation of cytosolic proteins in vivo. The proposed mechanism might explain not only the molecular basis of the toxic effects of cadmium but also the suggested role of this poisonous metal in the pathogenesis of certain protein-folding disorders.

zinc

protein degradation

protein folding

Saccharomyces cerevisiae

metal toxicity

protein aggregation

cadmium

Författare

Therese Jacobson

Göteborgs universitet

S. Priya

Indian Institute of Toxicology Research

S. K. Sharma

Indian Institute of Toxicology Research

S. Andersson

Göteborgs universitet

Stefan Jakobsson

Göteborgs universitet

R. Tanghe

Göteborgs universitet

A. Ashouri

Göteborgs universitet

Sahlgrenska akademin

Sebastien Rauch

Chalmers, Bygg- och miljöteknik, Vatten Miljö Teknik

P. Goloubinoff

Université de Lausanne

P. Christen

Universität Zürich

Markus J. Tamás

Göteborgs universitet

Molecular and Cellular Biology

0270-7306 (ISSN)

Vol. 37 17 UNSP e00490-16

Ämneskategorier

Biologiska vetenskaper

DOI

10.1128/mcb.00490-16