Rat–Mouse and Rat–Human Comparative Maps Based on Gene Homology and High-Resolution Zoo-FISH
Artikel i vetenskaplig tidskrift, 2001

The laboratory rat, Rattus norvegicus, and the laboratory mouse, Mus musculus, are key animal models in biomedical research. A deeper understanding of the genetic interrelationsships between Homo sapiens and these two rodent species is desirable for extending the usefulness of the animal models. We present comprehensive rat–human and rat–mouse comparative maps, based on 1090 gene homology assignments available for rat genes. Radiation hybrid, FISH, and zoo-FISH mapping data have been integrated to produce comparative maps that are estimated to comprise 83–100% of the conserved regions between rat and mouse and 66–82% of the conserved regions between rat and human. The rat–mouse zoo-FISH analysis, supported by data for individual genes, revealed nine previously undetected conserved regions compared to earlier reports. Since there is almost complete genome coverage in the rat–mouse comparative map, we conclude that it is feasible to make accurate predictions of gene positions in the rat based on gene locations in the mouse.

Chromosome Mapping

Chromosomes/genetics

Fluorescence

Radiation Hybrid Mapping

Chromosomes

In Situ Hybridization

Human/genetics

Mice

Humans

Evolution

Rats

Molecular

Animals

Genes/*genetics

Författare

Sven Nilsson

Göteborgs universitet

Institutionen för matematik, Matematisk statistik

Khalil Helou

Göteborgs universitet

Anna Walentinsson

Göteborgs universitet

Claude Szpirer

Olle Nerman

Institutionen för matematik, Matematisk statistik

Göteborgs universitet

Fredrik Ståhl

Göteborgs universitet

Genomics

0888-7543 (ISSN) 1089-8646 (eISSN)

Vol. 74 287-98

Ämneskategorier

Bioinformatik och systembiologi

Genetik

DOI

10.1006/geno.2001.6550