Quantification of the Intracellular Life Time of Water Molecules to Measure Transport Rates of Human Aquaglyceroporins.
Artikel i vetenskaplig tidskrift, 2017

Orthodox aquaporins are transmembrane channel proteins that facilitate rapid diffusion of water, while aquaglyceroporins facilitate the diffusion of small uncharged molecules such as glycerol and arsenic trioxide. Aquaglyceroporins play important roles in human physiology, in particular for glycerol metabolism and arsenic detoxification. We have developed a unique system applying the strain of the yeast Pichia pastoris, where the endogenous aquaporins/aquaglyceroporins have been removed and human aquaglyceroporins AQP3, AQP7, and AQP9 are recombinantly expressed enabling comparative permeability measurements between the expressed proteins. Using a newly established Nuclear Magnetic Resonance approach based on measurement of the intracellular life time of water, we propose that human aquaglyceroporins are poor facilitators of water and that the water transport efficiency is similar to that of passive diffusion across native cell membranes. This is distinctly different from glycerol and arsenic trioxide, where high glycerol transport efficiency was recorded.

Författare

Madelene Palmgren

Malin Hernebring

Göteborgs universitet

Stefanie Eriksson

Karin Elbing

Göteborgs universitet

Cecilia Geijer

Chalmers, Biologi och bioteknik, Industriell bioteknik

Samo Lasič

Peter Dahl

Göteborgs universitet

Jesper S Hansen

Daniel Topgaard

Karin Lindkvist-Petersson

Göteborgs universitet

Journal of Membrane Biology

0022-2631 (ISSN) 1432-1424 (eISSN)

Vol. 250 6 629-639

Ämneskategorier

Biokemi och molekylärbiologi

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1007/s00232-017-9988-4

PubMed

28914342