Quantification of the Intracellular Life Time of Water Molecules to Measure Transport Rates of Human Aquaglyceroporins.
Artikel i vetenskaplig tidskrift, 2017

Orthodox aquaporins are transmembrane channel proteins that facilitate rapid diffusion of water, while aquaglyceroporins facilitate the diffusion of small uncharged molecules such as glycerol and arsenic trioxide. Aquaglyceroporins play important roles in human physiology, in particular for glycerol metabolism and arsenic detoxification. We have developed a unique system applying the strain of the yeast Pichia pastoris, where the endogenous aquaporins/aquaglyceroporins have been removed and human aquaglyceroporins AQP3, AQP7, and AQP9 are recombinantly expressed enabling comparative permeability measurements between the expressed proteins. Using a newly established Nuclear Magnetic Resonance approach based on measurement of the intracellular life time of water, we propose that human aquaglyceroporins are poor facilitators of water and that the water transport efficiency is similar to that of passive diffusion across native cell membranes. This is distinctly different from glycerol and arsenic trioxide, where high glycerol transport efficiency was recorded.


Madelene Palmgren

Göteborgs universitet

Malin Hernebring

Lunds universitet

Stefanie Eriksson

Lunds universitet

Karin Elbing

Lunds universitet

Cecilia Geijer

Göteborgs universitet

Samo Lasič

CR Development AB

Peter Dahl

Göteborgs universitet

Jesper S Hansen

Lunds universitet

Daniel Topgaard

Lunds universitet

Karin Lindkvist-Petersson

Göteborgs universitet

Lunds universitet

Journal of Membrane Biology

0022-2631 (ISSN) 1432-1424 (eISSN)

Vol. 250 6 629-639


Biokemi och molekylärbiologi


Livsvetenskaper och teknik (2010-2018)





Mer information

Senast uppdaterat