Pathway for the biosynthesis of the pigment chrysogine by Penicillium chrysogenum
Artikel i vetenskaplig tidskrift, 2018
Chrysogine is a yellow pigment produced by Penicillium chrysogenum and other filamentous fungi. Although the pigment was first isolated in 1973, its biosynthetic pathway has so far not been resolved. Here, we show that deletion of the highly expressed nonribosomal peptide synthetase (NRPS) gene Pc21g12630 (chyA) resulted in a decrease in the production of chrysogine and 13 related compounds in the culture broth of P. chrysogenum. Each of the genes of the chyAcontaining gene cluster was individually deleted, and corresponding mutants were examined by metabolic profiling in order to elucidate their function. The data suggest that the NRPS ChyA mediates the condensation of anthranilic acid and alanine into the intermediate 2-(2-aminopropanamido)benzoic acid, which was verified by feeding experiments of a ΔchyA strain with the chemically synthesized product. The remainder of the pathway is highly branched, yielding at least 13 chrysogine-related compounds.
Författare
Annarita Viggiano
Rijksuniversiteit Groningen
O. Salo
Rijksuniversiteit Groningen
Hazrat Ali
National Institute for Biotechnology and Genetic Engineering Pakistan
Rijksuniversiteit Groningen
Wiktor Szymanski
Rijksuniversiteit Groningen
P. P. Lankhorst
DSM Biotechnology Centre
Yvonne Nygård
Chalmers, Biologi och bioteknik, Industriell bioteknik
Rijksuniversiteit Groningen
R. A. L. Bovenberg
Rijksuniversiteit Groningen
DSM Biotechnology Centre
Arnold J.M. Driessen
Rijksuniversiteit Groningen
Applied and Environmental Microbiology
0099-2240 (ISSN) 1098-5336 (eISSN)
Vol. 84 4 e02246-17Ämneskategorier
Biokemi och molekylärbiologi
Mikrobiologi
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
DOI
10.1128/AEM.02246-17