Rhamnolipids production from sucrose by engineered Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2018

Biosurfactants are biological tensioactive agents that can be used in the cosmetic and food industries. Rhamnolipids are glycolipid biosurfactants naturally produced by Pseudomonas aeruginosa and are composed of one or two rhamnose molecules linked to beta-hydroxy fatty acid chains. These compounds are green alternatives to petrochemical surfactants, but their large-scale production is still in its infancy, hindered due to pathogenicity of natural producer, high substrate and purification costs and low yields and productivities. This study, for the first time, aimed at producing mono-rhamnolipids from sucrose by recombinant GRAS Saccharomyces cerevisiae strains. Six enzymes from P. aeruginosa involved in mono-rhamnolipid biosynthesis were functionally expressed in the yeast. Furthermore, its SUC2 invertase gene was disrupted and a sucrose phosphorylase gene from Pelomonas saccharophila was also expressed to reduce the pathway's overall energy requirement. Two strains were constructed aiming to produce mono-rhamnolipids and the pathway's intermediate dTDP-L-rhamnose. Production of both molecules was analyzed by confocal microscopy and mass spectrometry, respectively. These strains displayed, for the first time as a proof of concept, the potential of production of these molecules by a GRAS eukaryotic microorganism from an inexpensive substrate. These constructs show the potential to further improve rhamnolipids production in a yeast-based industrial bioprocess.

Författare

Frederico Mendonça Bahia Silva

Universidade de Brasilia

Gabriela Carneiro de Almeida

Universidade Catolica de Brasilia

Lorena Pereira De Andrade

Universidade de Brasilia

Christiane Gonçalves Campos

Empresa Brasileira de Pesquisa Agropecuaria (Embrapa)

Universidade Federal de Goias

Lúcio Rezende Queiroz

Universidade de Brasilia

Rayane Luzia Vieira Da Silva

Universidade Catolica de Brasilia

Patrícia Verardi Abdelnur

Universidade Federal de Goias

Empresa Brasileira de Pesquisa Agropecuaria (Embrapa)

José Raimundo Corrêa

Universidade de Brasilia

Maurizio Bettiga

EviKrets Biobased Processes Consultants

Chalmers, Biologi och bioteknik, Industriell bioteknik

Nádia Skorupa-Parachin

Universidade de Brasilia

Scientific Reports

2045-2322 (ISSN)

Vol. 8 1 2905

Ämneskategorier

Biokemi och molekylärbiologi

Mikrobiologi

Biokatalys och enzymteknik

DOI

10.1038/s41598-018-21230-2

Mer information

Senast uppdaterat

2018-02-27