A new combined multicompartmental model for apolipoprotein B-100 and triglyceride metabolism in VLDL subfractions
Artikel i vetenskaplig tidskrift, 2005

The use of stable isotopes in conjunction with compartmental modeling analysis has greatly facilitated studies of the metabolism of the apolipoprotein B (apoB)-containing lipoproteins in humans. The aim of this study was to develop a multicompartment model that allows us to simultaneously determine the kinetics of apoB and triglyceride (TG) in VLDL(1) and VLDL(2) after a bolus injection of [(2)H(3)]leucine and [(2)H(5)]glycerol and to follow the catabolism and transfer of the lipoprotein particles. Here, we describe the model and present the results of its application in a fasting steady-state situation in 17 subjects with lipid values representative of a Western population. Analysis of the correlations showed that plasma TG was determined by the VLDL(1) and VLDL(2) apoB and TG fractional catabolic rate. Furthermore, the model showed a linear correlation between VLDL(1) TG and apoB production. A novel observation was that VLDL TG entered the circulation within 21 min after its synthesis, whereas VLDL apoB entered the circulation after 33 min. These observations are consistent with a sequential assembly model of VLDL and suggest that the TG is added to a primordial apoB-containing particle in the liver.

Triglycerides/biosynthesis/*metabolism

Deuterium/administration & dosage/metabolism

Models

Protein Transport

Kinetics

Glycerol/administration & dosage/metabolism

Lipoproteins

Biological

Humans

Apolipoproteins B/biosynthesis/*metabolism

VLDL/biosynthesis/*metabolism

Apolipoprotein B-100

Leucine/administration & dosage/metabolism

Författare

Martin Adiels

Göteborgs universitet

Chalmers, Matematiska vetenskaper

C. Packard

M. J. Caslake

P. Stewart

A. Soro

J. Westerbacka

Bernt Wennberg

Chalmers, Matematiska vetenskaper

Göteborgs universitet

Sven-Olof Olofsson

Göteborgs universitet

M. R. Taskinen

Jan Borén

Göteborgs universitet

Journal of Lipid Research

0022-2275 (ISSN) 1539-7262 (eISSN)

Vol. 46 58-67

Ämneskategorier

MEDICIN OCH HÄLSOVETENSKAP

DOI

10.1194/jlr.M400108-JLR200

PubMed

15489544