Spatiotemporal Kinetics of Supported Lipid Bilayer Formation on Glass via Vesicle Adsorption and Rupture
Artikel i vetenskaplig tidskrift, 2018

Supported lipid bilayers (SLBs) represent one of the most popular mimics of the cell membrane. Herein, we have used total internal reflection fluorescence microscopy for in-depth characterization of the vesicle-mediated SLB formation mechanism on a common silica-rich substrate, borosilicate glass. Fluorescently labeling a subset of vesicles allowed us to monitor the adsorption of individual labeled vesicles, resolve the onset of SLB formation from small seeds of SLB patches, and track their growth via SLB-edge-induced autocatalytic rupture of adsorbed vesicles. This made it possible to perform the first quantitative measurement of the SLB front velocity, which is shown to increase up to 1 order of magnitude with time. This effect can be classified as dramatic because in many other physical, chemical, or biological kinetic processes the front velocity is either constant or decreasing with time. The observation was successfully described with a theoretical model and Monte Carlo simulations implying rapid local diffusion of lipids upon vesicle rupture.

Författare

Mokhtar Mapar

Chalmers, Fysik, Biologisk fysik

Silver Jõemetsa

Chalmers, Fysik, Biologisk fysik

Hudson Pace

Chalmers, Fysik, Biologisk fysik

Vladimir Zhdanov

Chalmers, Fysik, Kemisk fysik

Björn Agnarsson

Chalmers, Fysik, Biologisk fysik

Fredrik Höök

Chalmers, Fysik, Biologisk fysik

Journal of Physical Chemistry Letters

1948-7185 (eISSN)

Vol. 9 5143-5149

Ämneskategorier

Fysikalisk kemi

Bioteknisk apparatteknik

Biofysik

DOI

10.1021/acs.jpclett.8b02092

PubMed

30137991

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Senast uppdaterat

2018-09-17