PrgB promotes aggregation, biofilm formation, and conjugation through DNA binding and compaction
Artikel i vetenskaplig tidskrift, 2018

Gram-positive bacteria deploy type IV secretion systems (T4SSs) to facilitate horizontal gene transfer. The T4SSs of Gram-positive bacteria rely on surface adhesins as opposed to conjugative pili to facilitate mating. Enterococcus faecalis PrgB is a surface adhesin that promotes mating pair formation and robust biofilm development in an extracellular DNA (eDNA) dependent manner. Here, we report the structure of the adhesin domain of PrgB. The adhesin domain binds and compacts DNA in vitro. In vivo PrgB deleted of its adhesin domain does not support cellular aggregation, biofilm development and conjugative DNA transfer. PrgB also binds lipoteichoic acid (LTA), which competes with DNA binding. We propose that PrgB binding and compaction of eDNA facilitates cell aggregation and plays an important role in establishment of early biofilms in mono- or polyspecies settings. Within these biofilms, PrgB mediates formation and stabilization of direct cell-cell contacts through alternative binding of cell-bound LTA, which in turn promotes establishment of productive mating junctions and efficient intra- or inter-species T4SS-mediated gene transfer.

Författare

Andreas Schmitt

Umeå universitet

Kai Jiang

Chalmers, Biologi och bioteknik, Kemisk biologi

Martha I. Camacho

University of Texas Medical School at Houston

Venkateswara Rao Jonna

Umeå universitet

Anders Hofer

Umeå universitet

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

Peter J. Christie

University of Texas Medical School at Houston

Ronnie Berntsson

Umeå universitet

Molecular Microbiology

0950-382X (ISSN) 1365-2958 (eISSN)

Vol. 109 3 291-305

Ämneskategorier

Cellbiologi

Biokemi och molekylärbiologi

Cell- och molekylärbiologi

DOI

10.1111/mmi.13980

Mer information

Senast uppdaterat

2018-09-13