Intracellular miRNA or siRNA delivery and function
Artikel i vetenskaplig tidskrift, 2018

Nanocarrier-mediated delivery and release of short non-coding RNA (e.g., miRNA or siRNA) into the cells with subsequent suppression of the populations of some of the mRNAs and proteins is of interest in the context of the development of a new generation of drugs. Bearing in mind such applications, the author shows the specifics of the corresponding transient kinetics by using three generic models without and with feedback resulting in bistability in the gene expression. In the absence of feedback, the suppression of the mRNA and protein population is transient. In the case of bistable kinetics, non-coding RNA can induce transition from the initial steady state to another steady state. The duration of this transition can be much longer than the time scale characterizing the drop of the non-coding RNA population. Quantitatively, the effect of the delivered non-coding RNA on gene expression can be appreciable if the maximum non-coding RNA population in the cytoplasm is comparable to or above 1000. All these conclusions have been drawn on the basis of calculations performed with the kinetic parameters typical for human cells.

Mean-field kinetic equations

Gene expression

Drug delivery

Subcellular processes

Författare

Vladimir Zhdanov

Chalmers, Fysik, Kemisk fysik

Russian Academy of Sciences

BioSystems

0303-2647 (ISSN)

Vol. 171 20-25

Ämneskategorier

Cellbiologi

Cell- och molekylärbiologi

Genetik

DOI

10.1016/j.biosystems.2018.05.007

PubMed

29859208

Mer information

Senast uppdaterat

2018-10-02