Characterization of different fat depots in NAFLD using inflammation-associated proteome, lipidome and metabolome
Artikel i vetenskaplig tidskrift, 2018

Non-alcoholic fatty liver disease (NAFLD) is recognized as a liver manifestation of metabolic syndrome, accompanied with excessive fat accumulation in the liver and other vital organs. Ectopic fat accumulation was previously associated with negative effects at the systemic and local level in the human body. Thus, we aimed to identify and assess the predictive capability of novel potential metabolic biomarkers for ectopic fat depots in non-diabetic men with NAFLD, using the inflammation-associated proteome, lipidome and metabolome. Myocardial and hepatic triglycerides were measured with magnetic spectroscopy while function of left ventricle, pericardial and epicardial fat, subcutaneous and visceral adipose tissue were measured with magnetic resonance imaging. Measured ectopic fat depots were profiled and predicted using a Random Forest algorithm, and by estimating the Area Under the Receiver Operating Characteristic curves. We have identified distinct metabolic signatures of fat depots in the liver (TAG50:1, glutamate, diSM18:0 and CE20:3), pericardium (N-palmitoyl-sphinganine, HGF, diSM18:0, glutamate, and TNFSF14), epicardium (sphingomyelin, CE20:3, PC38:3 and TNFSF14), and myocardium (CE20:3, LAPTGF-β1, glutamate and glucose). Our analyses highlighted non-invasive biomarkers that accurately predict ectopic fat depots, and reflect their distinct metabolic signatures in subjects with NAFLD.

Författare

Alen Lovric

Kungliga Tekniska Högskolan (KTH)

Marit Granér

Helsinki University Central Hospital

Elias Björnson

Wallenberg Lab.

Chalmers, Biologi och bioteknik, Systembiologi

Muhammad Arif

Kungliga Tekniska Högskolan (KTH)

Rui Benfeitas

Kungliga Tekniska Högskolan (KTH)

Kristofer Nyman

Helsinki University Central Hospital

Marcus Ståhlman

Wallenberg Lab.

Markku O. Pentikäinen

Helsinki University Central Hospital

J. Lundbom

Helsinki University Central Hospital

A. Hakkarainen

Helsinki University Central Hospital

Reijo Sirén

City of Helsinki

Markku S. Nieminen

Helsinki University Central Hospital

N. Lundbom

Helsinki University Central Hospital

Kirsi Lauerma

Helsinki University Central Hospital

Marja Riitta Taskinen

Helsinki University Central Hospital

Adil Mardinoglu

Chalmers, Biologi och bioteknik, Systembiologi

Kungliga Tekniska Högskolan (KTH)

Jan Borén

Wallenberg Lab.

Scientific Reports

2045-2322 (ISSN)

Vol. 8 1 14200

Ämneskategorier

Endokrinologi och diabetes

Annan klinisk medicin

Biologiska vetenskaper

Näringslära

Infrastruktur

Beräkningsinfrastruktur för systembiologi

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1038/s41598-018-31865-w

Mer information

Senast uppdaterat

2018-12-10