Lignin-first biomass fractionation using a hybrid organosolv – Steam explosion pretreatment technology improves the saccharification and fermentability of spruce biomass
Artikel i vetenskaplig tidskrift, 2019

For a transition to a sustainable society, fuels, chemicals, and materials should be produced from renewable resources. Lignocellulosic biomass constitutes an abundant and renewable feedstock; however, its successful application in a biorefinery requires efficient fractionation into its components; cellulose, hemicellulose and lignin. Here, we demonstrate that a newly established hybrid organosolv – steam explosion pretreatment can effectively fractionate spruce biomass to yield pretreated solids with high cellulose (72% w/w) and low lignin (delignification up to 79.4% w/w) content. The cellulose-rich pretreated solids present high saccharification yields (up to 61% w/w) making them ideal for subsequent bioconversion processes. Moreover, under high-gravity conditions (22% w/w) we obtained an ethanol titer of 61.7 g/L, the highest so far reported for spruce biomass. Finally, the obtained high-purity lignin is suitable for various advanced applications. In conclusion, hybrid organosolv pretreatment could offer a closed-loop biorefinery while simultaneously adding value to all biomass components.

High gravity fermentation




Organosolv explosion


Leonidas Matsakas

Luleå tekniska universitet

Vijayendran Raghavendran

Chalmers, Biologi och bioteknik, Industriell bioteknik

Olga Yakimenko

Luleå tekniska universitet

Gustav Persson

Chalmers, Fysik, Eva Olsson Group

Eva Olsson

Chalmers, Fysik, Eva Olsson Group

Ulrika Rova

Luleå tekniska universitet

Lisbeth Olsson

Chalmers, Biologi och bioteknik, Industriell bioteknik

P. Christakopoulos

Luleå tekniska universitet

Bioresource Technology

0960-8524 (ISSN) 1873-2976 (eISSN)

Vol. 273 521-528


Nanovetenskap och nanoteknik



Annan fysik



Chalmers materialanalyslaboratorium



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