Label-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells
Artikel i vetenskaplig tidskrift, 2019

Microorganisms adapt their biophysical properties in response to changes in their local environment. However, quantifying these changes at the single-cell level has only recently become possible, largely relying on fluorescent labeling strategies. In this work, we utilize yeast (Saccharomyces cerevisiae) to demonstrate label-free quantification of changes in both intracellular osmolarity and macromolecular concentration in response to changes in the local environment. By combining a digital holographic microscope with a millifluidic chip, the temporal response of cellular water flux was successfully isolated from the rate of production of higher molecular weight compounds, in addition to identifying the produced compounds in terms of the product of their refractive index increment (dndc) and molar mass. The ability to identify, quantify and temporally resolve multiple biophysical processes in living cells at the single cell level offers a crucial complement to label-based strategies, suggesting broad applicability in studies of a wide-range of cellular processes.

Författare

Daniel Midtvedt

Chalmers, Fysik, Biologisk fysik

Erik Olsén

Chalmers, Fysik, Biologisk fysik

Fredrik Höök

Chalmers, Fysik, Biologisk fysik

Gavin Jeffries

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Nature Communications

2041-1723 (ISSN)

Vol. 10 1 340

Ämneskategorier

Biokemi och molekylärbiologi

Annan medicinsk grundvetenskap

Mikrobiologi

DOI

10.1038/s41467-018-08207-5

PubMed

30664642

Mer information

Senast uppdaterat

2019-02-27