Hemocompatibility of siRNA loaded dextran nanogels
Artikel i vetenskaplig tidskrift, 2011
Although the behavior of nanoscopic delivery systems in blood is an important parameter when contemplating their intravenous injection, this aspect is often poorly investigated when advancing from in vitro to in vivo experiments. In this paper, the behavior of siRNA loaded dextran nanogels in human plasma and blood is examined using fluorescence fluctuation spectroscopy, platelet aggregometry, flow cytometry and single particle tracking. Our results show that, in contrast to their negatively charged counterparts, positively charged siRNA loaded dextran nanogels cause platelet aggregation and show increased binding to human blood cells. Although PEGylating the nanogels did not have a significant effect on their interaction with blood cells, single particle tracking revealed that it is necessary to prevent their aggregation in human plasma. We therefore conclude that PEGylated negatively charged dextran nanogels are the most suited for further in vivo studies as they do not aggregate in human plasma and exhibit minimal interactions with blood cells.
gene therapy
blood compatibility
haemocompatibility
blood
drug delivery
nanoparticle
Författare
B Naeye
Universiteit Gent
H Deschout
Universiteit Gent
Magnus Röding
Chalmers, Matematiska vetenskaper, Matematisk statistik
SuMo Biomaterials
Göteborgs universitet
Mats Rudemo
Chalmers, Matematiska vetenskaper, Matematisk statistik
Göteborgs universitet
J Delanghe
Center for Medical Genetics
K Devreese
Center for Medical Genetics
J Demeester
Universiteit Gent
K Braeckmans
Universiteit Gent
SC De Smedt
Universiteit Gent
K Raemdonck
Universiteit Gent
Biomaterials
0142-9612 (ISSN) 18785905 (eISSN)
Vol. 32 34 9120-9127Ämneskategorier
Biomaterialvetenskap
DOI
10.1016/j.biomaterials.2011.08.015
PubMed
21890194