Hemocompatibility of siRNA loaded dextran nanogels
Journal article, 2011

Although the behavior of nanoscopic delivery systems in blood is an important parameter when contemplating their intravenous injection, this aspect is often poorly investigated when advancing from in vitro to in vivo experiments. In this paper, the behavior of siRNA loaded dextran nanogels in human plasma and blood is examined using fluorescence fluctuation spectroscopy, platelet aggregometry, flow cytometry and single particle tracking. Our results show that, in contrast to their negatively charged counterparts, positively charged siRNA loaded dextran nanogels cause platelet aggregation and show increased binding to human blood cells. Although PEGylating the nanogels did not have a significant effect on their interaction with blood cells, single particle tracking revealed that it is necessary to prevent their aggregation in human plasma. We therefore conclude that PEGylated negatively charged dextran nanogels are the most suited for further in vivo studies as they do not aggregate in human plasma and exhibit minimal interactions with blood cells.

gene therapy

blood compatibility

haemocompatibility

blood

drug delivery

nanoparticle

Author

B Naeye

Ghent university

H Deschout

Ghent university

Magnus Röding

Chalmers, Mathematical Sciences, Mathematical Statistics

SuMo Biomaterials

University of Gothenburg

Mats Rudemo

Chalmers, Mathematical Sciences, Mathematical Statistics

University of Gothenburg

J Delanghe

Center for Medical Genetics

K Devreese

Center for Medical Genetics

J Demeester

Ghent university

K Braeckmans

Ghent university

SC De Smedt

Ghent university

K Raemdonck

Ghent university

Biomaterials

0142-9612 (ISSN)

Vol. 32 34 9120-9127

Subject Categories

Biomaterials Science

DOI

10.1016/j.biomaterials.2011.08.015

PubMed

21890194

More information

Latest update

8/18/2020