DNA polymerase η contributes to genome-wide lagging strand synthesis
Artikel i vetenskaplig tidskrift, 2019

DNA polymerase η (pol η) is best known for its ability to bypass UV-induced thymine-thymine (T-T) dimers and other bulky DNA lesions, but pol η also has other cellular roles. Here, we present evidence that pol η competes with DNA polymerases α and δ for the synthesis of the lagging strand genome-wide, where it also shows a preference for T-T in the DNA template. Moreover, we found that the C-terminus of pol η, which contains a PCNA-Interacting Protein motif is required for pol η to function in lagging strand synthesis. Finally, we provide evidence that a pol η dependent signature is also found to be lagging strand specific in patients with skin cancer. Taken together, these findings provide insight into the physiological role of DNA synthesis by pol η and have implications for our understanding of how our genome is replicated to avoid mutagenesis, genome instability and cancer.


Katrin Kreisel

Göteborgs universitet

Martin Engqvist

Göteborgs universitet

Chalmers, Biologi och bioteknik, Systembiologi

Josephine Kalm

Göteborgs universitet

Liam J. Thompson

Göteborgs universitet

Martin Boström

Göteborgs universitet

Clara Navarrete Roman

Göteborgs universitet

John P. McDonald

National Institute of Child Health and Human Development

Erik Larsson

Göteborgs universitet

Roger Woodgate

National Institute of Child Health and Human Development

Anders R. Clausen

Göteborgs universitet

Nucleic Acids Research

0305-1048 (ISSN) 1362-4962 (eISSN)

Vol. 47 5 2425-2435


Medicinsk biovetenskap

Biokemi och molekylärbiologi

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)





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