Anti-biofilm effects of gold and silver nanoparticles synthesized by the Rhodiola rosea rhizome extracts
Artikel i vetenskaplig tidskrift, 2018

Bacterial biofilm represents a major problem in medicine. They colonize and damage medical devices and implants and, in many cases, foster development of multidrug-resistant microorganisms. Biofilm development starts by bacterial attachment to the surface and the production of extracellular polymeric substances (EPS). The EPS forms a structural scaffold for dividing bacterial cells. The EPS layers also play a protective role, preventing the access of antibiotics to biofilm-associated microorganisms. The aim of this work was to investigate the production nanoparticles that could be used to inhibit biofilm formation. The applied production procedure from rhizome extracts of Rhodiola rosea is simple and environmentally friendly, as it requires no additional reducing, stabilizing and capping agents. The produced nanoparticles were stable and crystalline in nature with an average diameter of 13–17 nm for gold nanoparticles (AuNPs) and 15–30 nm for silver nanoparticles (AgNPs). Inductively coupled plasma mass spectrometry analysis revealed the concentration of synthesized nanoparticles as 3.3 and 5.3 mg/ml for AuNPs and AgNPs, respectively. Fourier-transform infrared spectroscopy detected the presence of flavonoids, terpenes and phenols on the nanoparticle surface, which could be responsible for reducing the Au and Ag salts to nanoparticles and further stabilizing them. Furthermore, we explored the AgNPs for inhibition of Pseudomonas aeruginosa and Escherichia coli biofilms. AgNPs exhibited minimum inhibitory concentrations of 50 and 100 µg/ml, against P. aeruginosa and E. coli, respectively. The respective minimum bactericidal concentrations were 100 and 200 µg/ml. These results suggest that using the rhizome extracts of the medicinal plant R. rosea represents a viable route for green production of nanoparticles with anti-biofilm effects.

R. rosea

silver nanoparticles

gold nanoparticles

E. coli

biofilm

P. aeruginosa

Författare

Priyanka Singh

Danmarks Tekniske Universitet (DTU)

Santosh Pandit

Chalmers, Biologi och bioteknik, Systembiologi

Mariam Beshay

Chalmers, Biologi och bioteknik, Systembiologi

Venkata Raghavendra Subrahmanya Sar Mokkapati

Chalmers, Kemi och kemiteknik, Energi och material, Oorganisk miljökemi 2

Jørgen Garnæs

Dansk Fundamental Metrologi AS

Mikael Emil Olsson

Danmarks Tekniske Universitet (DTU)

Abida Sultan

Danmarks Tekniske Universitet (DTU)

Aiga Mackevica

Danmarks Tekniske Universitet (DTU)

Ramona Valentina Mateiu

Danmarks Tekniske Universitet (DTU)

Henrik Lütken

Köpenhamns universitet

Anders Egede Daugaard

Danmarks Tekniske Universitet (DTU)

A. Baun

Danmarks Tekniske Universitet (DTU)

Ivan Mijakovic

Danmarks Tekniske Universitet (DTU)

Chalmers, Biologi och bioteknik, Systembiologi

Artificial Cells, Nanomedicine and Biotechnology

2169-1401 (ISSN) 2169-141X (eISSN)

Vol. 46 sup3 S886-S899

Ämneskategorier

Analytisk kemi

Annan kemi

Mikrobiologi

DOI

10.1080/21691401.2018.1518909

PubMed

30422688

Mer information

Senast uppdaterat

2019-08-22