Antibody−antigen interaction dynamics revealed by analysis of single-molecule equilibrium fluctuations on individual plasmonic nanoparticle biosensors
Artikel i vetenskaplig tidskrift, 2018

Antibody−antigen interactions are complex events central to immune response, in vivo and in vitro diagnostics, and development of therapeutic substances. We developed an ultrastable single-molecule localized surface plasmon resonance (LSPR) sensing platform optimized for studying antibody−antigen interaction kinetics over very long time scales. The setup allowed us to perform equilibrium fluctuations analysis of the PEG/anti-PEG interaction. By time and frequency domain analysis, we demonstrate that reversible adsorption of monovalently bound anti-PEG antibodies is the dominant factor affecting the LSPR fluctuations. The results suggest that equilibrium fluctuation analysis can be an alternative to established methods for determination of interaction rates. In particular, the methodology is suited to analyze molecular systems whose properties change during the initial interaction phases, for example, due to mass transport limitations or, as demonstrated here, because the effective association rate constant varies with surface concentration of adsorbed molecules.




Reaction rate constants


Equilibrium fluctuations


Srdjan Acimovic

Chalmers, Fysik, Bionanofotonik

Hana Jungová

Chalmers, Fysik, Bionanofotonik

Gustav Emilsson

Chalmers, Kemi och kemiteknik, Tillämpad kemi

Lei Shao

Chalmers, Fysik, Bionanofotonik

Andreas Dahlin

Chalmers, Kemi och kemiteknik, Tillämpad kemi

Mikael Käll

Chalmers, Fysik

Tomasz Antosiewicz

Uniwersytet Warszawski

Chalmers, Fysik, Bionanofotonik

ACS Nano

1936-0851 (ISSN) 1936-086X (eISSN)

Vol. 12 10 9958-9965


Fysikalisk kemi

Analytisk kemi

Den kondenserade materiens fysik





Mer information

Senast uppdaterat