Investigation of psoriasis skin tissue by label-free multi-modal imaging: a case study on a phototherapy-treated patient
Artikel i vetenskaplig tidskrift, 2019

Background: Psoriasis is a systemic inflammatory disease characterized by epidermal proliferation in the skin. Altered lipid metabolism is considered to be a central factor in the psoriatic etiopathogenesis. Thus, it is necessary to visualize chemical specificity of the samples for better medical diagnosis and treatment. Here, we investigate its role in the development of psoriatic lesions, before and after ultraviolet phototherapy, in a case study. Methods: The distribution and morphology of different lipids and fibrous proteins in psoriatic (lesional) tissues were visualized by two complementary label-free imaging techniques: 1) non-linear microscopy (NLM), providing images of lipids/proteins throughout the skin layers at submicrometer resolution; and 2) mass spectrometry imaging (MSI), offering high chemical specificity and hence the detection of different lipid species in the epidermal and dermal regions. A conventional method of histological evaluation was performed on the tissues, with no direct comparison with NLM and MSI. Results: Psoriatic tissues had a higher lipid content, mainly in cholesterol, in both the epidermal and dermal regions, compared to healthy tissues. Moreover, the collagen and elastin fibers in the psoriatic tissues had a tendency to assemble as larger bundles, while healthy tissues showed smaller fibers more homogeneously spread. Although phototherapy significantly reduced the cholesterol content, it also increased the amounts of collagen in both lesional and non-lesional tissues. Conclusion: This study introduces NLM and MSI as two complementary techniques which are chemical specific and can be used to assess and visualize the distribution of lipids, collagen, and elastin in a non-invasive and label-free manner.

collagen

psoriasis skin tissue

time-of-flight secondary ion mass spectrometry (ToF-SIMS)

lipids

non-linear microscopy

Författare

Nisha Rani Agarwal

Chalmers, Biologi och bioteknik, Kemisk biologi

Masoumeh Dowlatshahi Pour

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Maria Siekkeri Vandikas

Göteborgs universitet

Noora Neittaanmaki

Göteborgs universitet

Amra Osmancevic

Göteborgs universitet

Per Malmberg

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Psoriasis: Targets and Therapy

2230-326X (eISSN)

Vol. 9 43-57

Ämneskategorier

Medicinsk laboratorie- och mätteknik

Medicinsk bioteknik

Radiologi och bildbehandling

DOI

10.2147/PTT.S200366

PubMed

31410348

Mer information

Senast uppdaterat

2019-09-11