Gel Phase 1,2-Distearoyl- sn-glycero-3-phosphocholine-Based Liposomes Are Superior to Fluid Phase Liposomes at Augmenting Both Antigen Presentation on Major Histocompatibility Complex Class II and Costimulatory Molecule Display by Dendritic Cells in Vitro
Artikel i vetenskaplig tidskrift, 2019

Lipid-based nanoparticles have in recent years attracted increasing attention as pharmaceutical carriers. In particular, reports of them having inherent adjuvant properties combined with their ability to protect antigen from degradation make them suitable as vaccine vectors. However, the physicochemical profile of an ideal nanoparticle for vaccine delivery is still poorly defined. Here, we used an in vitro dendritic cell assay to assess the immunogenicity of a variety of liposome formulations as vaccine carriers and adjuvants. Using flow cytometry, we investigated liposome-assisted antigen presentation as well as the expression of relevant costimulatory molecules on the cell surface. Cytokine secretion was further evaluated with an enzyme-linked immunosorbent assay (ELISA). We show that liposomes can successfully enhance antigen presentation and maturation of dendritic cells, as compared to vaccine fusion protein (CTA1-3Eα-DD) administered alone. In particular, the lipid phase state of the membrane was found to greatly influence the vaccine antigen processing by dendritic cells. As compared to their fluid phase counterparts, gel phase liposomes were more efficient at improving antigen presentation. They were also superior at upregulating the costimulatory molecules CD80 and CD86 as well as increasing the release of the cytokines IL-6 and IL-1β. Taken together, we demonstrate that gel phase liposomes, while nonimmunogenic on their own, significantly enhance the antigen-presenting ability of dendritic cells and appear to be a promising way forward to improve vaccine immunogenicity.

mucosal vaccination

adjuvant effect

dendritic cell activation

immunogenicity

antigen delivery

lipid nanoparticle

Författare

Karin Norling

Chalmers, Fysik, Biologisk fysik

V. Bernasconi

Göteborgs universitet

V. A. Hernandez

Uppsala universitet

Nagma Parveen

Chalmers, Fysik, Biologisk fysik

KU Leuven

K. Edwards

Uppsala universitet

N. Y. Lycke

Göteborgs universitet

Fredrik Höök

Chalmers, Fysik, Biologisk fysik

Marta Bally

Umeå universitet

ACS Infectious Diseases

2373-8227 (eISSN)

Vol. 5 11 1867-1878

Ämneskategorier

Immunologi

Immunologi inom det medicinska området

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

DOI

10.1021/acsinfecdis.9b00189

PubMed

31498993

Mer information

Senast uppdaterat

2019-11-13