Natural Microbial Communities Can Be Manipulated by Artificially Constructed Biofilms
Artikel i vetenskaplig tidskrift, 2019

Biofouling proceeds in successive steps where the primary colonizers affect the phylogenetic and functional structure of a future microbial consortium. Using microbiologically influenced corrosion (MIC) as a study case, a novel approach for material surface protection is described, which does not prevent biofouling, but rather shapes the process of natural biofilm development to exclude MIC-related microorganisms. This approach interferes with the early steps of natural biofilm formation affecting how the community is finally developed. It is based on a multilayer artificial biofilm, composed of electrostatically modified bacterial cells, producing antimicrobial compounds, extracellular antimicrobial polyelectrolyte matrix, and a water-proof rubber elastomer barrier. The artificial biofilm is constructed layer-by-layer (LBL) by manipulating the electrostatic interactions between microbial cells and material surfaces. Field testing on standard steel coupons exposed in the sea for more than 30 days followed by laboratory analyses using molecular-biology tools demonstrate that the preapplied artificial biofilm affects the phylogenetic structure of the developing natural biofilm, reducing phylogenetic diversity and excluding MIC-related bacteria. This sustainable solution for material protection showcases the usefulness of artificially guiding microbial evolutionary processes via the electrostatic modification and controlled delivery of bacterial cells and extracellular matrix to the exposed material surfaces.

polyelectrolytes

bacteria

16S rRNA

layer-by-layer (LBL)

metagenomic

nanolayers

Författare

Tomaž Rijavec

Institute of Metagenomics and Microbial Technologies (IMMT)

Institut Jožef Stefan

Jan Zrimec

Chalmers, Biologi och bioteknik, Systembiologi

Institute of Metagenomics and Microbial Technologies (IMMT)

Rob van Spanning

Vrije Universiteit Amsterdam

Aleš Lapanje

Institut Jožef Stefan

Institute of Metagenomics and Microbial Technologies (IMMT)

Advanced Science

2198-3844 (ISSN)

Vol. In press 1901408

Ämneskategorier

Textil-, gummi- och polymermaterial

Materialkemi

Mikrobiologi

DOI

10.1002/advs.201901408

Mer information

Senast uppdaterat

2019-11-04