Determining the virulence properties of Escherichia coli ST131 containing bacteriocin-encoding plasmids using short-and long-read sequencing and comparing them with those of other E. Coli lineages
Artikel i vetenskaplig tidskrift, 2019

T. Escherichia coli ST131 is a clinical challenge due to its multidrug resistant profile and successful global spread. They are often associated with complicated infections, particularly urinary tract infections (UTIs). Bacteriocins play an important role to outcompete other microorganisms present in the human gut. Here, we characterized bacteriocin-encoding plasmids found in ST131 isolates of patients suffering from a UTI using both short-and long-read sequencing. Colicins Ia, Ib and E1, and microcin V, were identified among plasmids that also contained resistance and virulence genes. To investigate if the potential transmission range of the colicin E1 plasmid is influenced by the presence of a resistance gene, we constructed a strain containing a plasmid which had both the colicin E1 and blaCMY-2 genes. No difference in transmission range was found between transformant and wild-type strains. However, a statistically significantly difference was found in adhesion and invasion ability. Bacteriocin-producing isolates from both ST131 and non-ST131 lineages were able to inhibit the growth of other E. coli isolates, including other ST131. In summary, plasmids harboring bacteriocins give additional advantages for highly virulent and resistant ST131 isolates, improving the ability of these isolates to compete with other microbiota for a niche and thereby increasing the risk of infection.

E. Coli

Plasmids

Bacteriocins

ST131

UTIs

AmpC-beta-lactamase

Virulence

Författare

Ana Carolina da Cruz Campos

Rijksuniversiteit Groningen

Universidade do Estado do Rio de Janeiro

Francis M. Cavallo

Rijksuniversiteit Groningen

Nathália L. Andrade

Universidade do Estado do Rio de Janeiro

Jan Maarten van Dijl

Rijksuniversiteit Groningen

Natacha Couto

Rijksuniversiteit Groningen

Jan Zrimec

Chalmers, Biologi och bioteknik, Systembiologi

Jerome R.Lo Ten Foe

Rijksuniversiteit Groningen

Ana C.P. Rosa

Universidade do Estado do Rio de Janeiro

Paulo V. Damasco

Universidade Federal do Estado do Rio de Janeiro

Alex W. Friedrich

Rijksuniversiteit Groningen

Monika A. Chlebowicz-Flissikowska

Rijksuniversiteit Groningen

John W.A. Rossen

Rijksuniversiteit Groningen

Microorganisms

20762607 (eISSN)

Vol. 7 11 534

Ämneskategorier

Infektionsmedicin

Mikrobiologi

Mikrobiologi inom det medicinska området

DOI

10.3390/microorganisms7110534

Mer information

Senast uppdaterat

2019-11-26