Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis
Artikel i vetenskaplig tidskrift, 2019

The yeast Brettanomyces bruxellensis (syn. Dekkera bruxellensis) is an emerging and undesirable contaminant in industrial low-sugar ethanol fermentations that employ the yeast Saccharomyces cerevisiae. High-affinity glucose import in B. bruxellensis has been proposed to be the mechanism by which this yeast can outcompete S. cerevisiae. The present study describes the characterization of two B. bruxellensis genes (BHT1 and BHT3) believed to encode putative high-affinity glucose transporters. In vitro-generated transcripts of both genes as well as the S. cerevisiae HXT7 high-affinity glucose transporter were injected into Xenopus laevis oocytes and subsequent glucose uptake rates were assayed using 14C-labelled glucose. At 0.1 mM glucose, Bht1p was shown to transport glucose five times faster than Hxt7p. pH affected the rate of glucose transport by Bht1p and Bht3p, indicating an active glucose transport mechanism that involves proton symport. These results suggest a possible role for BHT1 and BHT3 in the competitive ability of B. bruxellensis.

yeast

glucose transport

Xenopus laevis

high-affinity

Brettanomyces bruxellensis

metabolism

bioethanol

Författare

Ievgeniia Tiukova

Chalmers, Biologi och bioteknik, Systembiologi

Iben Møller-Hansen

Danmarks Tekniske Universitet (DTU)

Zeinu M. Belew

Köpenhamns universitet

Behrooz Darbani

Danmarks Tekniske Universitet (DTU)

E Boles

Johann Wolfgang Goethe Universität Frankfurt am Main

Hussam H. Nour-Eldin

Köpenhamns universitet

Tomas Linder

Sveriges lantbruksuniversitet (SLU)

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

I. Borodina

Danmarks Tekniske Universitet (DTU)

FEMS microbiology letters

15746968 (eISSN)

Vol. 366 17

Ämneskategorier

Biokemi och molekylärbiologi

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

Annan industriell bioteknik

DOI

10.1093/femsle/fnz222

PubMed

31665273

Mer information

Senast uppdaterat

2019-11-26