Identification and characterisation of two high-affinity glucose transporters from the spoilage yeast Brettanomyces bruxellensis
Journal article, 2019

The yeast Brettanomyces bruxellensis (syn. Dekkera bruxellensis) is an emerging and undesirable contaminant in industrial low-sugar ethanol fermentations that employ the yeast Saccharomyces cerevisiae. High-affinity glucose import in B. bruxellensis has been proposed to be the mechanism by which this yeast can outcompete S. cerevisiae. The present study describes the characterization of two B. bruxellensis genes (BHT1 and BHT3) believed to encode putative high-affinity glucose transporters. In vitro-generated transcripts of both genes as well as the S. cerevisiae HXT7 high-affinity glucose transporter were injected into Xenopus laevis oocytes and subsequent glucose uptake rates were assayed using 14C-labelled glucose. At 0.1 mM glucose, Bht1p was shown to transport glucose five times faster than Hxt7p. pH affected the rate of glucose transport by Bht1p and Bht3p, indicating an active glucose transport mechanism that involves proton symport. These results suggest a possible role for BHT1 and BHT3 in the competitive ability of B. bruxellensis.

Xenopus laevis

Brettanomyces bruxellensis





glucose transport


Ievgeniia Tiukova

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Iben Møller-Hansen

Technical University of Denmark (DTU)

Zeinu M. Belew

University of Copenhagen

Behrooz Darbani

Technical University of Denmark (DTU)

E Boles

Goethe University Frankfurt

Hussam H. Nour-Eldin

University of Copenhagen

Tomas Linder

Swedish University of Agricultural Sciences (SLU)

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

I. Borodina

Technical University of Denmark (DTU)

FEMS microbiology letters

03781097 (ISSN) 15746968 (eISSN)

Vol. 366 17

Subject Categories

Biochemistry and Molecular Biology

Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Other Industrial Biotechnology





More information

Latest update

7/2/2021 1