Mirror-Image 5S Ribonucleoprotein Complexes
Artikel i vetenskaplig tidskrift, 2019

After realizing mirror-image genetic replication, transcription, and reverse transcription, the biggest challenge in establishing a mirror-image version of the central dogma is to build a mirror-image ribosome-based translation machine. Here, we chemically synthesized the natural and mirror-image versions of three ribosomal proteins (L5, L18, and L25) in the large subunit of the Escherichia coli ribosome with post-translational modifications. We show that the synthetic mirror-image proteins can fold in vitro despite limited efficiency and assemble with enzymatically transcribed mirror-image 5S ribosomal RNA into ribonucleoprotein complexes. In addition, the RNA-protein interactions are chiral-specific in that the mirror-image ribosomal proteins do not bind with natural 5S ribosomal RNA and vice versa. The synthesis and assembly of mirror-image 5S ribonucleoprotein complexes are important steps towards building a functional mirror-image ribosome.

native chemical ligation

chemical protein synthesis

ribonucleoproteins

chirality

solid-phase peptide synthesis

Författare

Jun-Jie Ling

Tsinghua University

Chuyao Fan

Tsinghua University

Hong Qin

Tsinghua University

Min Wang

Tsinghua University

Ji Chen

Tsinghua University

Pernilla Wittung Stafshede

Chalmers, Biologi och bioteknik, Kemisk biologi

Ting F. Zhu

Tsinghua University

Angewandte Chemie - International Edition

1433-7851 (ISSN) 1521-3773 (eISSN)

Vol. In Press

Ämneskategorier

Biokemi och molekylärbiologi

Strukturbiologi

Datorseende och robotik (autonoma system)

DOI

10.1002/anie.201914799

PubMed

31841243

Mer information

Senast uppdaterat

2020-02-10