Absolute yeast mitochondrial proteome quantification reveals trade-off between biosynthesis and energy generation during diauxic shift
Artikel i vetenskaplig tidskrift, 2020

Saccharomyces cerevisiae constitutes a popular eukaryal model for research on mitochondrial physiology. Being Crabtree-positive, this yeast has evolved the ability to ferment glucose to ethanol and respire ethanol once glucose is consumed. Its transition phase from fermentative to respiratory metabolism, known as the diauxic shift, is reflected by dramatic rearrangements of mitochondrial function and structure. To date, the metabolic adaptations that occur during the diauxic shift have not been fully characterized at the organelle level. In this study, the absolute proteome of mitochondria was quantified alongside precise parametrization of biophysical properties associated with the mitochondrial network using state-of-the-art optical-imaging techniques. This allowed the determination of absolute protein abundances at a subcellular level. By tracking the transformation of mitochondrial mass and volume, alongside changes in the absolute mitochondrial proteome allocation, we could quantify how mitochondria balance their dual role as a biosynthetic hub as well as a center for cellular respiration. Furthermore, our findings suggest that in the transition from a fermentative to a respiratory metabolism, the diauxic shift represents the stage where major structural and functional reorganizations in mitochondrial metabolism occur. This metabolic transition, initiated at the mitochondria level, is then extended to the rest of the yeast cell.

Diauxic shift

Absolute proteomics

Saccharomyces cerevisiae



Francesca Di Bartolomeo

Sintef Foundation for Scientific and Industrial Research At the Norwegian Institute of Technology

Chalmers, Biologi och bioteknik, Systembiologi

Carl Malina

Chalmers, Biologi och bioteknik, Systembiologi

Kate Campbell

Chalmers, Biologi och bioteknik, Systembiologi

Maurizio Mormino

Chalmers, Biologi och bioteknik, Industriell bioteknik

Johannes Fuchs

Göteborgs universitet

Egor Vorontsov

Göteborgs universitet

Claes M Gustafsson

Göteborgs universitet

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

BioInnovation Institute

Danmarks Tekniske Universitet (DTU)

Proceedings of the National Academy of Sciences of the United States of America

0027-8424 (ISSN) 1091-6490 (eISSN)

Vol. 117 13 7524-7535


Biokemi och molekylärbiologi


Annan fysik





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