Absolute yeast mitochondrial proteome quantification reveals trade-off between biosynthesis and energy generation during diauxic shift
Journal article, 2020

Saccharomyces cerevisiae constitutes a popular eukaryal model for research on mitochondrial physiology. Being Crabtree-positive, this yeast has evolved the ability to ferment glucose to ethanol and respire ethanol once glucose is consumed. Its transition phase from fermentative to respiratory metabolism, known as the diauxic shift, is reflected by dramatic rearrangements of mitochondrial function and structure. To date, the metabolic adaptations that occur during the diauxic shift have not been fully characterized at the organelle level. In this study, the absolute proteome of mitochondria was quantified alongside precise parametrization of biophysical properties associated with the mitochondrial network using state-of-the-art optical-imaging techniques. This allowed the determination of absolute protein abundances at a subcellular level. By tracking the transformation of mitochondrial mass and volume, alongside changes in the absolute mitochondrial proteome allocation, we could quantify how mitochondria balance their dual role as a biosynthetic hub as well as a center for cellular respiration. Furthermore, our findings suggest that in the transition from a fermentative to a respiratory metabolism, the diauxic shift represents the stage where major structural and functional reorganizations in mitochondrial metabolism occur. This metabolic transition, initiated at the mitochondria level, is then extended to the rest of the yeast cell.

Diauxic shift

Absolute proteomics

Saccharomyces cerevisiae

Mitochondria

Author

Francesca Di Bartolomeo

Sintef Foundation for Scientific and Industrial Research At the Norwegian Institute of Technology

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Carl Malina

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Kate Campbell

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Maurizio Mormino

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Johannes Fuchs

University of Gothenburg

Egor Vorontsov

University of Gothenburg

Claes M Gustafsson

University of Gothenburg

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

BioInnovation Institute

Technical University of Denmark (DTU)

Proceedings of the National Academy of Sciences of the United States of America

0027-8424 (ISSN) 1091-6490 (eISSN)

Vol. 117 13 7524-7535

Subject Categories

Biochemistry and Molecular Biology

Physiology

Other Physics Topics

DOI

10.1073/pnas.1918216117

PubMed

32184324

More information

Latest update

5/8/2020 7