Bioprospecting Through Cloning of Whole Natural Product Biosynthetic Gene Clusters
Reviewartikel, 2020

Since the discovery of penicillin, natural products and their derivatives have been a valuable resource for drug discovery. With recent development of genome mining approaches in the post-genome era, a great number of natural product biosynthetic gene clusters (BGCs) have been identified and these can potentially be exploited for the discovery of novel natural products that can find application as pharmaceuticals. Since many BGCs are silent or do not express in native hosts under laboratory conditions, heterologous expression of BGCs in genetically tractable hosts becomes an attractive route to activate these BGCs to discover the corresponding products. Here, we highlight recent achievements in cloning and discovery of natural product biosynthetic pathways via intact BGC capturing, and discuss the prospects of high-throughput and multiplexed cloning of rational-designed gene clusters in the future.

natural product

biosynthetic gene cluster

direct cloning

sequence-independent cloning

heterologous expression

Författare

Zhenquan Lin

Beijing University of Chemical Technology

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

BioInnovation Institute

Danmarks Tekniske Universitet (DTU)

Beijing University of Chemical Technology

Zihe Liu

Beijing University of Chemical Technology

Frontiers in Bioengineering and Biotechnology

2296-4185 (ISSN)

Vol. 8 526

Ämneskategorier

Produktionsteknik, arbetsvetenskap och ergonomi

Mikrobiologi

Bioinformatik och systembiologi

DOI

10.3389/fbioe.2020.00526

Mer information

Senast uppdaterat

2020-08-07