Long-term in vivo integrity and safety of3D-bioprinted cartilaginous constructs
Artikel i vetenskaplig tidskrift, 2020

Long-term stability and biological safety are crucial for translation of 3D-bioprinting technology into clinical applications. Here, we addressed the long-term safety and stability issues associated with 3D-bioprinted constructs comprising a cellulose scaffold and human cells (chondrocytes and stem cells) over a period of 10 months in nude mice. Our findings showed that increasing unconfined compression strength over time significantly improved the mechanical stability of the cell-containing constructs relative to cell-free scaffolds. Additionally, the cell-free constructs exhibited a mean compressive stress and stiffness (compressive modulus) of 0.04 +/- 0.05 MPa and 0.14 +/- 0.18 MPa, respectively, whereas these values for the cell-containing constructs were 0.11 +/- 0.08 MPa (p= .019) and 0.53 +/- 0.59 MPa (p= .012), respectively. Moreover, histomorphologic analysis revealed that cartilage formed from the cell-containing constructs harbored an abundance of proliferating chondrocytes in clusters, and after 10 months, resembled native cartilage. Furthermore, extension of the experiment over the complete lifecycle of the animal model revealed no signs of ossification, fibrosis, necrosis, or implant-related tumor development in the 3D-bioprinted constructs. These findings confirm the in vivo biological safety and mechanical stability of 3D-bioprinted cartilaginous tissues and support their potential translation into clinical applications.

nude mice

cartilage

in vivo

chondrocytes

long-term

3D-bioprinting

Författare

Peter Apelgren

Göteborgs universitet

Matteo Amoroso

Göteborgs universitet

Karin Saljo

Göteborgs universitet

Anders Lindahl

Sahlgrenska universitetssjukhuset

Camilla Brantsing

Sahlgrenska universitetssjukhuset

Linnea Strid Orrhult

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Paul Gatenholm Group

Kajsa Markstedt

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Paul Gatenholm Group

Paul Gatenholm

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Paul Gatenholm Group

Lars Kolby

Göteborgs universitet

Journal of Biomedical Materials Research - Part B Applied Biomaterials

1552-4973 (ISSN)

Vol. In Press

Ämneskategorier

Cellbiologi

Cell- och molekylärbiologi

Biomaterialvetenskap

DOI

10.1002/jbm.b.34687

PubMed

32633102

Mer information

Senast uppdaterat

2020-09-17