Independent Size and Fluorescence Emission Determination of Individual Biological Nanoparticles Reveals that Lipophilic Dye Incorporation Does Not Scale with Particle Size
Artikel i vetenskaplig tidskrift, 2020

Advancements in nanoparticle characterization techniques are critical for improving the understanding of how biological nanoparticles (BNPs) contribute to different cellular processes, such as cellular communication, viral infection, as well as various drug-delivery applications. Since BNPs are intrinsically heterogeneous, there is a need for characterization methods that are capable of providing information about multiple parameters simultaneously, preferably at the single-nanoparticle level. In this work, fluorescence microscopy was combined with surface-based two-dimensional flow nanometry, allowing for simultaneous and independent determination of size and fluorescence emission of individual BNPs. In this way, the dependence of the fluorescence emission of the commonly used self-inserting lipophilic dye 3,3′-dioctadecyl-5,5′-di(4-sulfophenyl)oxacarbocyanine (SP-DiO) could successfully be correlated with nanoparticle size for different types of BNPs, including synthetic lipid vesicles, lipid vesicles derived from cellular membrane extracts, and extracellular vesicles derived from human SH-SY5Y cell cultures; all vesicles had a radius, r, of ∼50 nm and similar size distributions. The results demonstrate that the dependence of fluorescence emission of SP-DiO on nanoparticle size varies significantly between the different types of BNPs, with the expected dependence on membrane area, r2, being observed for synthetic lipid vesicles, while a significant weaker dependence on size was observed for BNPs with more complex composition. The latter observation is attributed to a size-dependent difference in membrane composition, which may influence either the optical properties of the dye and/or the insertion efficiency, indicating that the fluorescence emission of this type of self-inserting dye may not be reliable for determining size or size distribution of BNPs with complex lipid compositions.

microfluidics

Lipids

Membranes

Fluorescence

Vesicles

Nanoparticles

Microscopy

Författare

Silver Jõemetsa

Chalmers, Fysik, Biologisk fysik

Paul Joyce

Chalmers, Fysik, Biologisk fysik

Quentin Lubart

Chalmers, Biologi och bioteknik, Kemisk biologi

Mokhtar Mapar

Chalmers, Fysik, Biologisk fysik

Emanuele Celauro

Chalmers, Biologi och bioteknik, Kemisk biologi

Björn Agnarsson

Chalmers, Fysik, Nano- och biofysik

Stephan Block

Chalmers, Fysik, Biologisk fysik

Marta Bally

Chalmers, Fysik, Biologisk fysik

Elin Esbjörner Winters

Chalmers, Biologi och bioteknik, Kemisk biologi

Gavin Jeffries

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Fredrik Höök

Chalmers, Fysik, Nano- och biofysik

Langmuir

07437463 (ISSN) 15205827 (eISSN)

Vol. 36 33 9693-9700

Ämneskategorier

Fysikalisk kemi

Materialkemi

Annan fysik

Biofysik

Nanoteknik

DOI

10.1021/acs.langmuir.0c00941

PubMed

32787069

Mer information

Senast uppdaterat

2020-09-17