Bilberry Supplementation after Myocardial Infarction Decreases Microvesicles in Blood and Affects Endothelial Vesiculation
Artikel i vetenskaplig tidskrift, 2020

Scope: Diet rich in bilberries is considered cardioprotective, but the mechanisms of action are poorly understood. Cardiovascular disease is characterized by increased proatherogenic status and high levels of circulating microvesicles (MVs). In an open-label study patients with myocardial infarction receive an 8 week dietary supplementation with bilberry extract (BE). The effect of BE on patient MV levels and its influence on endothelial vesiculation in vitro is investigated. Methods and results: MVs are captured with acoustic trapping and platelet-derived MVs (PMVs), as well as endothelial-derived MVs (EMVs) are quantified with flow cytometry. The in vitro effect of BE on endothelial extracellular vesicle (EV) release is examined using endothelial cells and calcein staining. The mechanisms of BE influence on vesiculation pathways are studied by Western blot and qRT-PCR. Supplementation with BE decreased both PMVs and EMVs. Furthermore, BE reduced endothelial EV release, Akt phosphorylation, and vesiculation-related gene transcription. It also protects the cells from P2X7-induced EV release and increase in vesiculation-related gene expression. Conclusion: BE supplementation improves the MV profile in patient blood and reduces endothelial vesiculation through several molecular mechanisms related to the P2X7 receptor. The findings provide new insight into the cardioprotective effects of bilberries.

bilberries

microvesicles

cardiovascular diseases

P2X (P2X purinoreceptor 7) 7

Författare

Paulina Bryl-Górecka

Lunds universitet

Ramasri Sathanoori

Lunds universitet

Lilith Arevström

Örebro universitet

Rikard Landberg

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Cecilia Bergh

Örebro universitet

Mikael Evander

Lunds universitet

Björn Olde

Lunds universitet

Thomas Laurell

Lunds universitet

Ole Fröbert

Örebro universitet

David Erlinge

Lunds universitet

Molecular Nutrition and Food Research

1613-4125 (ISSN)

Vol. In Press

Ämneskategorier

Annan klinisk medicin

Cell- och molekylärbiologi

Hematologi

DOI

10.1002/mnfr.202000108

PubMed

32846041

Mer information

Senast uppdaterat

2020-10-23