ToF-SIMS imaging of dual biomolecular monolayer gradients
Artikel i vetenskaplig tidskrift, 2020

Precise characterization of a monolayer of two different biomolecules in a gradient pattern on a glass surface puts high demand on the method used. Some techniques can detect protein monolayers but not on a glass surface. Others can distinguish between different proteins but not identify a gradient pattern. Here, we used ToF-SIMS to validate the complete surface composition, checking all the necessary boxes. As these types of surfaces can dictate sensitive cell behaviors, the precision on a nanolevel is crucial, and to visualize and determine the molecular distribution become essential. The dual monolayer consisted of laminin 521 and one of three other biomolecules of different sizes, epidermal growth factor, growth differentiation factor 5, or bovine serum albumin, creating opposing gradient patterns. The resulting ToF-SIMS imaging and line scan data provided detailed information on the distribution of the adsorbed proteins.

Författare

Hanne Evenbratt

Cline Scientific

Marwa Munem

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Per Malmberg

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Biointerphases

1559-4106 (ISSN) 1934-8630 (eISSN)

Vol. 15 6 061014

Ämneskategorier

Fysikalisk kemi

Bioteknisk apparatteknik

Biofysik

Styrkeområden

Hälsa och teknik

Infrastruktur

Infrastruktur för kemisk avbildning

Chalmers materialanalyslaboratorium

DOI

10.1116/6.0000621

PubMed

33353309

Mer information

Senast uppdaterat

2021-01-14