Putative metabolites involved in the beneficial effects of wholegrain cereal: Nontargeted metabolite profiling approach
Artikel i vetenskaplig tidskrift, 2021

Background and aims: Wholegrain cereals have been implicated in the reduction of lifestyle-related chronic diseases risk including cardiovascular diseases and type 2 diabetes. Molecular mechanisms responsible for the beneficial health effects are not entirely understood. The aims of this study were 1) to identify new potential plasma biomarker candidate metabolites of wholegrain cereal foods intake and 2) to examine whether some putative metabolites associated with wholegrain foods intake may play a role in the improvement of cardiometabolic risk factors. Methods and results: Analysis have been conducted in 54 individuals with metabolic syndrome of both genders, age 40–65 years, randomly assigned to 2 dietary interventions lasting 12-week: 1) wholegrain enriched diet (n = 28), and 2) refined-wheat cereals diet (control diet) (n = 26). Nontargeted metabolite profiling analysis was performed on fasting plasma samples collected at baseline and at the end of the experimental diets. Our data show that, at the end of the intervention, a higher intake of wholegrain (tertile 3) was significantly associated with a marked increase in several lipid compounds, as PC (20:4/16:1), LPC (20:4), LPC (22:6), LPC (18:3), LPC (22:5), and a phenolic compound (P < .05 for all). In the wholegrain group, higher concentrations of these metabolites (tertile 3 vs tertile 1 of each metabolite) were significantly associated with lower postprandial insulin and triglyceride responses (P < .05) by 29% and 37%, respectively. Conclusion: These observations suggest a possible role of lipid and polyphenol metabolites in the postprandial metabolic benefits of wholegrains in subjects at high risk of cardiovascular disease. In addition, they provide insight into the role of these metabolites as potential candidate biomarkers of wholegrain foods. The study was registered on ClinicalTrials.gov (identifier: NCT00945854).

Wholegrain cereals

Metabolic effect

Dietary biomarker


Nontargeted metabolite profiling


Marilena Vitale

Universita degli Studi di Napoli Federico II

Kati Hanhineva

Turun Yliopisto

Itä-Suomen Yliopisto

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Ville Mikael Koistinen

Itä-Suomen Yliopisto

Seppo Auriola

Itä-Suomen Yliopisto

Jussi Paananen

Itä-Suomen Yliopisto

Giuseppina Costabile

Universita degli Studi di Napoli Federico II

Giuseppe Della Pepa

Universita degli Studi di Napoli Federico II

Angela A. Rivellese

Universita degli Studi di Napoli Federico II

Gabriele Riccardi

Universita degli Studi di Napoli Federico II

Rosalba Giacco

Consiglo Nazionale Delle Richerche

Nutrition, Metabolism and Cardiovascular Diseases

0939-4753 (ISSN) 1590-3729 (eISSN)

Vol. 31 4 1156-1165


Endokrinologi och diabetes

Annan klinisk medicin






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