A novel stepwise integrative analysis pipeline reveals distinct microbiota-host interactions and link to symptoms in irritable bowel syndrome
Artikel i vetenskaplig tidskrift, 2021

Although incompletely understood, microbiota-host interactions are assumed to be altered in irritable bowel syndrome (IBS). We, therefore, aimed to develop a novel analysis pipeline tailored for the integrative analysis of microbiota-host interactions and association to symptoms and prove its utility in a pilot cohort. A multilayer stepwise integrative analysis pipeline was developed to visualize complex variable associations. Application of the pipeline was demonstrated on a dataset of IBS patients and healthy controls (HC), using the R software package to analyze colonic host mRNA and mucosal microbiota (16S rRNA gene sequencing), as well as gastrointestinal (GI) and psychological symptoms. In total, 42 IBS patients (57% female, mean age 33.6 (range 18–58)) and 20 HC (60% female, mean age 26.8 (range 23–41)) were included. Only in IBS patients, mRNA expression of Toll-like receptor 4 and genes associated with barrier function (PAR2, OCLN, TJP1) intercorrelated closely, suggesting potential functional relationships. This host genes-based “permeability cluster” was associated to mucosa-adjacent Chlamydiae and Lentisphaerae, and furthermore associated to satiety as well as to anxiety, depression and fatigue. In both IBS patients and HC, chromogranins, secretogranins and TLRs clustered together. In IBS patients, this host genes-based “immune-enteroendocrine cluster” was associated to specific members of Firmicutes, and to depression and fatigue, whereas in HC no significant association to microbiota was identified. We have developed a stepwise integrative analysis pipeline that allowed identification of unique host-microbiota intercorrelation patterns and association to symptoms in IBS patients. This analysis pipeline may aid in advancing the understanding of complex variable associations in health and disease.


A. Polster

Sahlgrenska universitetssjukhuset

Lena Öhman

Sahlgrenska universitetssjukhuset

Göteborgs universitet

Julien Tap

Groupe Danone

Muriel Derrien

Groupe Danone

B. Le Nevé

Groupe Danone

Johanna Sundin

Göteborgs universitet

Sahlgrenska universitetssjukhuset

H. Törnblom

Sahlgrenska universitetssjukhuset

Marija Cvijovic

Göteborgs universitet

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

M. Simren

The University of North Carolina at Chapel Hill

Sahlgrenska universitetssjukhuset

Scientific Reports

2045-2322 (ISSN) 20452322 (eISSN)

Vol. 11 1 5521



Medicinsk genetik

Reumatologi och inflammation





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