Dynamics of Ku and bacterial non-homologous end-joining characterized using single DNA molecule analysis
Artikel i vetenskaplig tidskrift, 2021

We use single-molecule techniques to characterize the dynamics of prokaryotic DNA repair by non-homologous end-joining (NHEJ), a system comprised only of the dimeric Ku and Ligase D (LigD). The Ku homodimer alone forms a ∼2 s synapsis between blunt DNA ends that is increased to ∼18 s upon addition of LigD, in a manner dependent on the C-terminal arms of Ku. The synapsis lifetime increases drastically for 4 nt complementary DNA overhangs, independently of the C-terminal arms of Ku. These observations are in contrast to human Ku, which is unable to bridge either of the two DNA substrates. We also demonstrate that bacterial Ku binds the DNA ends in a cooperative manner for synapsis initiation and remains stably bound at DNA junctions for several hours after ligation is completed, indicating that a system for removal of the proteins is active in vivo. Together these experiments shed light on the dynamics of bacterial NHEJ in DNA end recognition and processing. We speculate on the evolutionary similarities between bacterial and eukaryotic NHEJ and discuss how an increased understanding of bacterial NHEJ can open the door for future antibiotic therapies targeting this mechanism.

Författare

Robin Öz

Chalmers, Biologi och bioteknik, Kemisk biologi

Jing L. Wang

Ecole Normale Superieure (ENS)

Institut Jacques Monod

Raphael Guerois

Université Paris-Saclay

Gaurav Goyal

Chalmers, Biologi och bioteknik, Kemisk biologi

Sriram Kesarimangalam

Chalmers, Biologi och bioteknik, Kemisk biologi

Virginie Ropars

Université Paris-Saclay

Rajhans Sharma

Chalmers, Biologi och bioteknik, Kemisk biologi

Firat Koca

Student vid Chalmers

Jean Baptiste Charbonnier

Université Paris-Saclay

M. Modesti

Equipe Labélisée

Centre de Recherche en Cancerologie de Marseille

Terence R. Strick

Equipe Labélisée

Institut Jacques Monod

Ecole Normale Superieure (ENS)

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

Nucleic Acids Research

0305-1048 (ISSN) 1362-4962 (eISSN)

Vol. 49 5 2629-2641

Ämneskategorier

Biofysik

Mikrobiologi

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

DOI

10.1093/nar/gkab083

PubMed

33590005

Mer information

Senast uppdaterat

2021-04-28