Antimicrobial Peptide-Functionalized Mesoporous Hydrogels
Artikel i vetenskaplig tidskrift, 2021

Antimicrobial peptides (AMPs) are seen as a promising replacement to conventional antibiotics for the prevention of skin wound infections. However, due to the short half-life of AMPs in biological environments, such as blood, their use in clinical applications has been limited. The covalent immobilization of AMPs onto suitable substrates is an effective solution to create contact-killing surfaces with increased long-term stability. In this work, an antimicrobial peptide, RRPRPRPRPWWWW-NH2 (RRP9W4N), was covalently attached to amphiphilic and ordered mesoporous Pluronic F127 hydrogels made of cross-linked lyotropic liquid crystals through 1-ethyl-3-(3-(dimethylamino)propyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry. The AMP-hydrogels showed high antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, methicillin-resistant S. aureus (MRSA), and multidrug-resistant Escherichia coli for up to 24 h. Furthermore, the AMP-hydrogels did not present any toxicity to human fibroblasts. The AMPs retained their antimicrobial activity up to 48 h in human blood serum, which is a significant increase in stability compared to when used in dissolved state. A pilot in vivo rat model showed 10-100x less viable counts of S. aureus on AMP-hydrogels compared with control hydrogels during the first 3 days of infection. Studies performed on human whole blood showed that blood coagulated more readily in the presence of AMP-hydrogels as compared to hydrogels without AMPs, indicating potential hemostatic activity. Overall, the results suggest that the combination of amphiphilic hydrogels with covalently bonded AMPs has potential to be used as antibacterial wound dressing material to reduce infections and promote hemostatic activity as an alternative to antibiotics or other antimicrobial agents, whose use should be restricted.

infection

hydrogels

prevention

antimicrobial peptides

Författare

Saba Atefyekta

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Martin Andersson Group

Edvin Blomstrand

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Martin Andersson Group

Anand Kumar Rajasekharan

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Martin Andersson Group

Sara Svensson

Göteborgs universitet

Margarita Trobos

Göteborgs universitet

Jaan Hong

Uppsala universitet

Thomas J. Webster

Northeastern University

Peter Thomsen

Göteborgs universitet

Martin Andersson

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Martin Andersson Group

ACS Biomaterial Science and Engineering

2373-9878 (eISSN)

Vol. 7 4 1693-1702

Ämneskategorier

Infektionsmedicin

Farmakologi och toxikologi

Biomaterialvetenskap

Styrkeområden

Materialvetenskap

DOI

10.1021/acsbiomaterials.1c00029

PubMed

33719406

Mer information

Senast uppdaterat

2021-05-24