Reconstruction of a Genome-Scale Metabolic Model of Streptomyces albus J1074: Improved Engineering Strategies in Natural Product Synthesis
Artikel i vetenskaplig tidskrift, 2021

Streptomyces albus J1074 is recognized as an effective host for heterologous production of natural products. Its fast growth and efficient genetic toolbox due to a naturally minimized genome have contributed towards its advantage in expressing biosynthetic pathways for a diverse repertoire of products such as antibiotics and flavonoids. In order to develop precise model-driven engineering strategies for de novo production of natural products, a genome-scale metabolic model (GEM) was reconstructed for the microorganism based on protein homology to model species Streptomyces coelicolor while drawing annotated data from databases and literature for further curation. To demonstrate its capabilities, the Salb-GEM was used to predict overexpression targets for desirable compounds using flux scanning with enforced objective function (FSEOF). Salb-GEM was also utilized to investigate the effect of a minimized genome on metabolic gene essentialities in comparison to another Streptomyces species, S. coelicolor.

gene essentiality

natural products

minimized genome

genome-scale metabolic model

heterologous production

Streptomyces albus J1074

Författare

Cheewin Kittikunapong

Chalmers, Biologi och bioteknik, Systembiologi

Suhui Ye

Universidad de Oviedo

Patricia Magadan-Corpas

Universidad de Oviedo

Alvaro Perez-Valero

Universidad de Oviedo

Claudio J. Villar

Universidad de Oviedo

Felipe Lombo

Universidad de Oviedo

Eduard Kerkhoven

Chalmers, Biologi och bioteknik, Systembiologi

Metabolites

2218-1989 (ISSN)

Vol. 11 5 304

Ämneskategorier

Mikrobiologi

Bioinformatik (beräkningsbiologi)

Bioinformatik och systembiologi

DOI

10.3390/metabo11050304

PubMed

34064751

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Senast uppdaterat

2021-06-22