Interbase-FRET binding assay for pre-microRNAs
Artikel i vetenskaplig tidskrift, 2021

The aberrant expression of microRNAs (miRs) has been linked to several human diseases. A promising approach for targeting these anomalies is the use of small-molecule inhibitors of miR biogenesis. These inhibitors have the potential to (i) dissect miR mechanisms of action, (ii) discover new drug targets, and (iii) function as new therapeutic agents. Here, we designed Forster resonance energy transfer (FRET)-labeled oligoribonucleotides of the precursor of the oncogenic miR-21 (pre-miR-21) and used them together with a set of aminoglycosides to develop an interbase-FRET assay to detect ligand binding to pre-miRs. Our interbase-FRET assay accurately reports structural changes of the RNA oligonucleotide induced by ligand binding. We demonstrate its application in a rapid, qualitative drug candidate screen by assessing the relative binding affinity between 12 aminoglycoside antibiotics and pre-miR-21. Surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) were used to validate our new FRET method, and the accuracy of our FRET assay was shown to be similar to the established techniques. With its advantages over SPR and ITC owing to its high sensitivity, small sample size, straightforward technique and the possibility for high-throughput expansion, we envision that our solution-based method can be applied in pre-miRNA-target binding studies.

Författare

Mattias Bood

Göteborgs universitet

AstraZeneca R&D

Anna Wiktoria Wypijewska Del Nogal

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Jesper Nilsson

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Fredrik Edfeldt

AstraZeneca AB

Anders Dahlen

AstraZeneca AB

Malin Lemurell

AstraZeneca AB

Marcus Wilhelmsson

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Morten Grotli

Göteborgs universitet

Scientific Reports

2045-2322 (ISSN)

Vol. 11 1 9396

DrugSearchTool

Europeiska kommissionen (EU) (EC/H2020/753595), 2018-09-01 -- 2020-08-31.

Utveckling och användning av FRET-prober - Avslöja ny, unik struktur- och dynamikinformation om nukleinsyrasystem

Vetenskapsrådet (VR) (2013-4375), 2014-01-01 -- 2017-12-31.

Styrkeområden

Nanovetenskap och nanoteknik (SO 2010-2017, EI 2018-)

Hälsa och teknik

Ämneskategorier

Biokemi och molekylärbiologi

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

Läkemedelskemi

DOI

10.1038/s41598-021-88922-0

PubMed

33931703

Mer information

Senast uppdaterat

2021-07-07