Vascularization of tissue engineered cartilage - Sequential in vivo MRI display functional blood circulation
Artikel i vetenskaplig tidskrift, 2021

Establishing functional circulation in bioengineered tissue after implantation is vital for the delivery of oxygen and nutrients to the cells. Native cartilage is avascular and thrives on diffusion, which in turn depends on proximity to circulation. Here, we investigate whether a gridded three-dimensional (3D) bioprinted construct would allow ingrowth of blood vessels and thus prove a functional concept for vascularization of bioengineered tissue. Twenty 10 × 10 × 3-mm 3Dbioprinted nanocellulose constructs containing human nasal chondrocytes or cell-free controls were subcutaneously implanted in 20 nude mice. Over the next 3 months, the mice were sequentially imaged with a 7 T small-animal MRI system, and the diffusion and perfusion parameters were analyzed. The chondrocytes survived and proliferated, and the shape of the constructs was well preserved. The diffusion coefficient was high and well preserved over time. The perfusion and diffusion patterns shown by MRI suggested that blood vessels develop over time in the 3D bioprinted constructs; the vessels were confirmed by histology and immunohistochemistry. We conclude that 3D bioprinted tissue with a gridded structure allows ingrowth of blood vessels and has the potential to be vascularized from the host. This is an essential step to take bioengineered tissue from the bench to clinical practice.



In vivo





3D bioprinting


Peter Apelgren

Sahlgrenska universitetssjukhuset

Göteborgs universitet

Matteo Amoroso

Göteborgs universitet

Sahlgrenska universitetssjukhuset

Karin Säljö

Göteborgs universitet

Sahlgrenska universitetssjukhuset

Mikael Montelius

Göteborgs universitet

Anders Lindahl

Göteborgs universitet

Linnea Strid Orrhult

Chalmers, Kemi och kemiteknik, Tillämpad kemi

Paul Gatenholm

Chalmers, Kemi och kemiteknik, Tillämpad kemi

Lars Kölby

Göteborgs universitet

Sahlgrenska universitetssjukhuset


0142-9612 (ISSN) 18785905 (eISSN)

Vol. 276 121002


Cell- och molekylärbiologi

Bioteknisk apparatteknik

Medicinsk bioteknik





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