Optical dna mapping of plasmids reveals clonal spread of carbapenem-resistant klebsiella pneumoniae in a large thai hospital
Artikel i vetenskaplig tidskrift, 2021

Carbapenem-resistant Klebsiella pneumoniae (CR-KP) in patients admitted to hospitals pose a great challenge to treatment. The genes causing resistance to carbapenems are mostly found in plasmids, mobile genetic elements that can spread easily to other bacterial strains, thus exacerbating the problem. Here, we studied 27 CR-KP isolates collected from different types of samples from 16 patients admitted to the medical ward at Siriraj Hospital in Bangkok, Thailand, using next generation sequencing (NGS) and optical DNA mapping (ODM). The majority of the isolates belonged to sequence type (ST) 16 and are described in detail herein. Using ODM, we identified the plasmid carrying the blaNDM-1 gene in the ST16 isolates and the plasmids were very similar, highlighting the possibility of using ODM of plasmids as a surrogate marker of nosocomial spread of bacteria. We also demonstrated that ODM could identify that the blaCTX-M-15 and blaOXA-232 genes in the ST16 isolates were encoded on separate plasmids from the blaNDM-1 gene and from each other. The other three isolates belonged to ST147 and each of them had distinct plasmids encoding blaNDM-1 .

Bla NDM-1

Optical DNA mapping

Nosocomial transmission


Carbapenem resistance


Sriram Kesarimangalam

Chalmers, Biologi och bioteknik, Kemisk biologi

Tsegaye Sewunet

Karolinska Institutet

Walaiporn Wangchinda

Siriraj Hospital

Teerawit Tangkoskul

Siriraj Hospital

Visanu Thamlikitkul

Siriraj Hospital

Christian G. Giske

Karolinska Institutet

Karolinska universitetssjukhuset

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi


2079-6382 (eISSN)

Vol. 10 9 1029




Mikrobiologi inom det medicinska området







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