Graphene coated magnetic nanoparticles facilitate the release of biofuels and oleochemicals from yeast cell factories
Artikel i vetenskaplig tidskrift, 2021

Engineering of microbial cells to produce high value chemicals is rapidly advancing. Yeast, bacteria and microalgae are being used to produce high value chemicals by utilizing widely available carbon sources. However, current extraction processes of many high value products from these cells are time- and labor-consuming and require toxic chemicals. This makes the extraction processes detrimental to the environment and not economically feasible. Hence, there is a demand for the development of simple, effective, and environmentally friendly method for the extraction of high value chemicals from these cell factories. Herein, we hypothesized that atomically thin edges of graphene having ability to interact with hydrophobic materials, could be used to extract high value lipids from cell factories. To achieve this, array of axially oriented graphene was deposited on iron nanoparticles. These coated nanoparticles were used to facilitate the release of intracellular lipids from Yarrowia lipolytica cells. Our treatment process can be integrated with the growth procedure and achieved the release of 50% of total cellular lipids from Y. lipolytica cells. Based on this result, we propose that nanoparticles coated with axially oriented graphene could pave efficient, environmentally friendly, and cost-effective way to release intracellular lipids from yeast cell factories.

Författare

Santosh Pandit

Chalmers, Biologi och bioteknik, Systembiologi

Oliver Konzock

Chalmers, Biologi och bioteknik, Systembiologi

Kirsten Leistner

The MathWorks AB

Venkata Raghavendra Subrahmanya Sar Mokkapati

Chalmers, Biologi och bioteknik, Systembiologi

Alessandra Merlo

Chalmers, Biologi och bioteknik, Systembiologi

Jie Sun

Chalmers, Mikroteknologi och nanovetenskap (MC2), Kvantkomponentfysik

Fuzhou University

Ivan Mijakovic

Danmarks Tekniske Universitet (DTU)

Chalmers, Biologi och bioteknik, Systembiologi

Scientific Reports

2045-2322 (ISSN)

Vol. 11 1 20612

Ämneskategorier

Cellbiologi

Annan kemi

Medicinska material och protesteknik

DOI

10.1038/s41598-021-00189-7

Mer information

Senast uppdaterat

2021-11-22